血管活性肠肽诱导裸鼠体内激素依赖性前列腺癌血管生成的研究

Hormone-dependent angiogenesis induced by vasoactive intestinal peptide in nude mouse prostate cancer LNCaP

目的 检测裸鼠体内激素依赖性前列腺癌（LNCaP）组织中的血管内皮生长因子（VEGF） mRNA含量和微血管密度（MVD）,探讨血管活性肠肽（VIP）促血管生成作用的机制.方法 利用雄性小鼠建立经100 nmol/L VIP和未经VIP处理的LNCaP动物模型,前者为实验组,后者为对照组.于第8天和第15天测量移植瘤的体积,利用实时定量聚合酶链反应（Real-time PCR,SYBR染料法）检测移植瘤组织中的VEGF mRNA水平,利用CD34计算移植瘤组织中的MVD.结果 第8天和第15天裸鼠LNCaP移植瘤体积、VEGF mRNA含量、MVD实验组[（55.38±10.82）、（89.41±15.66） mm3;1.38±0.41、1.76 ±0.67;43.7±11.4、59.1±13.8]均大于对照组[（28.56 ±7.39）、（48.73±12.48） mm3;0.42 ±0.13、0.59 ±0.24;26.3 ±9.2、30.6± 10.5],差异均有统计学意义（P＜0.05）.结论 VIP对裸鼠LNCaP移植瘤生长起促进作用,可能与其促进肿瘤新生血管相关.

Objective To explore the mechanism of vasoactive intestinal peptide （VIP） inducing angiogenesis by detecting the vascular endothelial growth factor （VEGF） mRNA content and microvessel density （MVD） in experimental prostate cancer in vivo.Methods Nude mice were subcutaneously injected with LNCaP prostate cancer cells.Cells were incubated for 1 h in the presence （experimental group） or absence （control group） of 100 nmol/L VIP for 1 h before xenograf.By measuring the transplanted tumor volume,real-time quantitative polymerase chain reaction （Real-time PCR） was used to detect VEGF mRNA,and CD34 was applied to calculate the MVD in the transplanted tumor tissue at 8th day and 15th day.Results The tumor volume,VEGF mRNA content and MVD in the experimental group [（55.38 ± 10.82）,（89.41 ±15.66） mm3; 1.38±0.41,1.76 ±0.67; 43.7±11.4,59.1 ± 13.8] were significantly increased as compared with those in the control group [（28.56 ±7.39）,（48.73 ± 12.48） mm3 ;0.42±0.13,0.59±0.24; 26.3 ±9.2,30.6±10.5; P＜0.05].Conclusion This study indicatesVIP promotes the growth of LNCaP cells probably by inducing angiogenesis in vivo.