禾谷炭疽菌RGS蛋白生物信息学分析

Bioinformatics analysis on regulators of G-protein signaling in Colletotrichum graminicola

【目的】明确禾谷炭疽菌中存在的典型RGS,及其信号肽、跨膜区、二级结构特征,明确该菌RGS与其他病菌之间的关系,最终为深入开展RGS定位、功能研究打下坚实的理论基础,也为进一步开展其他炭疽菌的研究提供重要的理论指导。【方法】基于酿酒酵母中已经报道的4个典型RGS序列,利用BLASTp以及关键词对禾谷炭疽菌蛋白质数据库进行比对、搜索,以及通过SMART保守结构域分析。同时,通过对禾谷炭疽菌中典型RGS氨基酸序列进行细胞信号肽、跨膜区结构以及二级结构等生物信息学分析,此外,通过对禾谷炭疽菌中的典型RGS与其他物种中的同源序列进行遗传关系比较分析。【结果】明确禾谷炭疽菌存在6个典型的RGS,上述RGS在蛋白质二级结构中均含有较高比例的α螺旋结构,而在信号肽方面,除CgRGS6含有明显的信号肽序列外,其他RGS则没有;6个RGS中3个定位在细胞核中,其他则定位在质膜、内质网、线粒体上。【结论】禾谷炭疽菌中的RGS与C.higginsianum、C.gloeosporioides Cg-14/Nara gc5、C.orbiculare等炭疽菌属中的病菌具有较高的同源序列,以及较近的亲缘关系。

[Objective] To determine the typical RGS in Colletotrichum graminicola, and its signal peptide, transmembrane region and the secondary structure, and to provide a strong theoretical foundation for studying the positioning and function of RGS, but also provide an important theoretical guidance to clarify the other pathogen in Colletotrichum spp.. [Methods] Based on the four typical RGS sequences have been reported in Saccharomyces cerevisiae, to search RGS related protein sequence from the protein databases of Colletotrichum spp. with the BLASTp as well as the use of words, and to search the conserved domain in the SMART online. Meanwhile, bioinformatics analysis have made including the signal peptide, trans-membrane domain structure and the secondary structure, In addition, analysis of genetic relationships through comparative typical RGS in C. graminicola with homologous sequences of other species. [Results] There are six typical RGSs in C. grarninicola, which have higher proportion of helical secondary structure. And these RGS do not contain the typical signal peptide sequence except for CgRGS6, and the half of six RGSs positioned in the nucleus, and the other is positioned in the plasma membrane, endoplasmic reticulum, and mitochondria, respectively. [Conclusion] C. higginsianum, C. gloeosporioides Cg-14/Nara gc5, C. orbiculare has a high sequence homology and close genetic relationship with C. graminicola.