黄芪多糖对免疫抑制肉鸡生长性能、肠道菌群及免疫功能的影响

Effect of Astragalus Polysaccharin on Growth Performance,Intestinal Microflora and Immune Function of Immunosuppression Broilers

本试验旨在探讨日粮中添加不同水平黄芪多糖对免疫抑制肉鸡生长性能、肠道微生物菌群及免疫功能的影响。选用180只体重相近的健康快大型黄羽肉杂鸡,随机分成4组,每组3个重复,每个重复15只鸡,Ⅰ组饲喂基础日粮,自由饮水;Ⅱ、Ⅲ、Ⅳ组在饮水中添加水溶性氟苯尼考200mg/L,用药6d后,Ⅱ组饲喂基础日粮,Ⅲ、Ⅳ组分别饲喂在基础日粮中添加500和1000mg/kg黄芪多糖的日粮。预试期7d,正试期35d。结果表明,与Ⅱ组相比,Ⅲ、Ⅳ组能显著改善免疫抑制肉鸡生长性能(P<0.05),增加免疫抑制肉鸡肠道中有益菌(乳酸杆菌、双歧杆菌)的数量(P<0.05),降低有害菌(大肠杆菌、沙门氏菌)的数量(P<0.05),提高免疫抑制肉鸡免疫器官指数及新城疫抗体效价(P<0.05),降低免疫抑制肉鸡血清中IL-10和IFN-γ含量(P<0.05)。综上所述,日粮中添加500和1000mg/kg黄芪多糖均能消除氟苯尼考诱导的免疫抑制作用,改善肉鸡生长性能,调节肉鸡肠道的微生态平衡,提高机体免疫功能。

The aim of this study was to explore the effect of different levels of Astragalus polysaccharin on growth performance,intestinal microflora and immune function of immunosuppression broilers.A total of 180 fast large yellow feather chickens with similar weight were randomly divided into 4 groups,with 3 replicates of 15 chicks in each replicate,groupⅠ was fed with basal diet,free drinking water,groupsⅡ,Ⅲ,Ⅳ were add 200mg/L water soluble florfenicol in the drinking water,medication for 6days,then groupⅡ was fed a basal diet,groupsⅢ,Ⅳ were fed with basal diet supplemented with 500 and 1000mg/kg Astragalus polysaccharin,respectively.The pre-trial period lasted for 7days,and the experiment lasted for 35 days.The results showed that compared with groupⅡ,groupsⅢandⅣ was significantly improved the growth performance of immunosuppression broilers（P〈0.05）,enhanced the number of beneficial bacteria（Lactobacillus and Bifidobacterium）in the gastrointestinal tract of immunosuppression broilers（P〈0.05）,reduced the number of harmful bacteria（Escherichia coli and Salmonella）in the gastrointestinal tract of immunosuppression broilers（P〈0.05）,improved the immune organs index and Newcastle disease antibody titer of immunosuppression broilers（P〈0.05）,decreased the serum contents of IL-10 and IFN-γof immunosuppression broilers（P〈0.05）.In conclusion,adding 500 and 1000mg/kg Astragalus polysaccharin in diet could eliminate immunosuppression effect of florfenicol test,improve the growth performance,adjust the intestinal micro ecological balance and ennhance the immune function of broilers.