山羊腹泻粪便中奇异变形杆菌的分离鉴定及系统发育分析

Isolation,Identification and Phylogenetic Analysis of Proteus mirabilis from Goats with Diarrhea

该研究从四川成都某羊场腹泻简阳大耳山羊粪便中分离鉴定出奇异变形杆菌（14/20）,随机选取5个菌株腹腔注射小鼠,5×108 CFU/只,小鼠于接种后12h内全部死亡。PCR扩增分离株的16SrRNA序列,测序结果显示其与GenBank中奇异变形杆菌的序列同源性为99%-100%,分离菌株间的同源性为99.8%-100%;系统发育分析结果显示,分离菌株与不同宿主源奇异变形杆菌共聚为2个分支,存在一定的多样性,但其进化不存在显著的地域及宿主相关性。本研究结果表明,奇异变形杆菌与山羊腹泻密切相关,对养羊业的危害及公共卫生学意义值得关注。

Proteus mirabilis were identified from goat manure samples with diarrhea from a goat farm in Chengdu of Sichuan province（14/20）.5isolates（5×108 CFU each）were randomly selected from 17 Proteus mirabilis and infected into mice by intraperitoneal injection.The results showed that all of the injected mice were dead within 12 h.16S rRNA genes of Proteus mirabilis isolates were sequenced and analyzed.The genes shared 99% to 100%homology with the sequence of Proteus mirabilis available in GenBank,and shared a homology of 99.8% to 100% within isolates in this study.Phylogenetic analysis showed that isolates clustered into two branches and presented some diversity,but no significant correlation of evolution between different areas and hosts.In conclusion,Proteus mirabilis was closely involved in goat diarrhea.Its harm to the sheep industry and public health significance should be seriously concerned.