腐殖酸钠对大肠杆菌的抑制作用研究

Study on Bacteriostatic Action of Sodium Humate on Escherichia coli

为探讨腐殖酸钠对大肠杆菌的抑制作用机理,试验采用稀释平板计数法测定不同水平腐殖酸钠对大肠杆菌的抑制效果,并通过透射电镜对大肠杆菌菌体进行观察。结果表明,添加5%、10%、15%腐殖酸钠可使大肠杆菌活菌数量明显降低(P<0.05);同一菌株随腐殖酸钠添加量增加,抑制效果明显加强(P<0.05);不同菌株在相同处理条件下,随时间的延长,抑制作用越明显。透射电镜下观察发现,添加5%腐殖酸钠组与添加5%腐殖酸钠调节pH组均可使大肠杆菌细胞膜通透性改变,细胞内出现空泡。综上所述,腐殖酸钠可有效抑制大肠杆菌生长。

The role of sodium humate on Escherichia coli was investigated to provide potential novel antibacterial drugs for treating disease which caused by enteropathogenic bacteria.Antimicrobial activity for sodium humate on Escherichia coli was determined using dilution plate counting method.Ultrastructure of sodium humate against Escherichia coli was observed under transmission electron microscope.The results showed that adding sodium humate（5%,10%,15%）decreased the number of Escherichia coli（P〈0.05）.The more obvious inhibition effect（P〈0.05）as the level of sodium humate content increased.Different strains in the same processing conditions,with the extension of time,the more obvious inhibitory effect.Observation under transmission electron microscope was carried out that adding 5% sodium humate and adding 5%sodium humate with pH regulating changed the permeability of cell membrane,made cell excrescent and inhibited cell division.The results provided a theoretical basis to further understand sterilization mechanism of sodium humate.