VRIN治疗后人胃癌裸鼠原位移植瘤活体成像观察

Bioluminescence imaging evaluation of the inhibitory effect of Ginsenoside Rg_3 immune-nanoemulsion(VRIN)on nude mice bearing gastric cancer cell lines

通过慢病毒转染构建稳定表达红色荧光蛋白(RFP)的NUGC-4细胞株,建立胃癌裸鼠移植瘤模型。活体成像系统观察肿瘤的生长情况;观察人参皂苷免疫纳米(VRIN)治疗后肿瘤体积及重量的变化;HE染色观察肿瘤的病理形态学变化。结果表明:建立稳定表达RFP胃癌细胞系及裸鼠移植瘤模型,活体荧光成像显示,治疗组裸鼠荧光强度较生理盐水对照组明显减弱;治疗组肿瘤体积及重量明显小于对照组(P<0.01),治疗组的肿瘤组织可见大片肿瘤细胞坏死,对照组肿瘤细胞坏死少。说明VRIN对人胃癌细胞祼鼠移植瘤生长有明显的抑制作用。

NUGC-4 cell lines with stable expression of red fluorescent protein （red fluorescent protein, RFP） were established by transfeetion via lentiviral vector. The gastric cancer models were developed by surgical orthotopic implantation of red fluorescent protein （RFP）-expressing NUGC-4 tumor in nude mice. The dynamic growth of xenografted tumor was observed using fluorescence imaging system in vivo. After treated with VEGFR3-mediated immune-nanoemulsion of ginsenoside Rg3 （VRIN）, the volume and weight of transplanted tumors in nude mice were measured. Morphology of transplanted tumor tissues was observed by HE staining. Results showed that stable RFP-expressing gastric cancer cell lines and xenografted tumor model were obtained. Optical imaging showed that the fluorescent intensity of VRIN treated group was apparently lower than that of the control group which was treated with saline. As compared to the control group, the volume and weight of tumors in VRIN treated group were significantly decreased （P〈0. 01）. VRIN treated group induced obvious necrosis of tumor cells in implanted tumor tissues. VRIN can inhibit the growth of human gastric cancer cell transplantation tumor in nude mice.