补阳还五汤对大鼠断肢再植术后肢体缺血再灌注损伤的影响

The Effect of Buyanghuanwu Decoction on Limb Replantation of Limb Ischemia Reperfusion Injury in Rats

目的:探讨补阳还五汤在大鼠下肢断肢再植术后缺血-再灌注损伤中的影响,评估其对缺血组织的保护作用及机制。方法:建立大鼠下肢断肢再植术后缺血-再灌注损伤动物模型,随机分为正常对照组、补阳还五汤组、依达拉奉组、空白对照组,测定血液NO水平、腓肠肌组织中超氧化物歧化酶(Superoxide Dismutase,SOD)活性、丙二醛(Maleic Dialdehyde,MDA)浓度以及钙离子负载;腓肠肌组织提取总RNA,采用RT-PCR半定量分析Bcl-2-mRNA和Bax-mRNA转录水平,观察腓肠肌组织形态学变化。结果:与其他组相比,补阳还五汤能有效降低肢体缺血再灌注后氧自由基产生,增强SOD活性,减轻钙超载所导致肢体缺血再灌注损伤;能够减少血浆NO的大量生成;通过增强抗凋亡基因Bcl-2的表达和减弱促进凋亡基因Bax表达抑制肢体缺血再灌注后骨骼肌细胞的凋亡,减轻肢体缺血再灌注损伤。结论:补阳还五汤对大鼠断肢再植术后下肢缺血-再灌注损伤具有明显的保护作用,其机制可能与其抗氧化、清除自由基、抑制骨骼肌细胞凋亡有关。

Objective： To investigate the effect of Buyanghuanwu decoction in rats with injury of lower limbs after replantation of severed limbs ischemia reperfusion, evaluate the protective effect and mechanism of ischemic tissue. Methods： Established the model of the isehemieal reperfusion injury in rats, randomly divided into four groups, the normal group,the Buyanghuanwu decoction group, the edaravone group, the vacuity contrast group, valuated the NO level in the blood, the activity of SOD in gastrocnemius tissue, the density of MDA and the load of Ca2＋; extracted the total RNA in gastrocnemius tissue, and analyzed the transcriptional level of Bcl-2-mRNA and Bax-mRNA by the RT-PCR semiquantitative; and observed the histomorphology change of the gastrocnemius tissue. Results： The Buyanghuanwu decoction could protect the rats from ischemieal reperfusion injury by lessening the calcium overload, reinforcing the activity of the SOD, getting rid of the oxygen free radical, up-regulating the express of the Bcl-2 and down-regulating the express of Bax,and inhibiting the apoptosis of the skeletal muscles. Conclusion： Buyanghuanwu decoction has significant protection on cerebral ischemiareperfusion injury in rats, which may be related to the inhibition of inflammatory cytokine secretion and expression and inducing the inflammation in gastrocnemius tissue.