多倍体啤酒酵母URA3基因的失活与恢复

Inactivation and Recovery of URA3 Gene of Polyploid Brewer's Yeast

以多倍体啤酒酵母菌株S6作为出发菌株,利用同源重组的方法构建了尿嘧啶营养缺陷型啤酒酵母菌株S6-ΔU,使之用于遗传标记,并把缺陷型菌株恢复成原营养型S6'。同时对出发菌株S6,缺陷型菌株S6-ΔU和恢复后的菌株S6'的生长状况进行比较。结果表明,URA3点突变并没有弱化菌株的生长状况。从而建立一种快速获得缺陷型啤酒酵母菌株的方法。

Polyploid brewer＇s yeast strain S6 was used as the starting strain to construct uracil auxotrophic strain S6-△U through gene homologous recombination, which could be used as a genetic marker. Then uracil auxotrophic strain S6-△U was restored to original strain and named strain S6＇. The growth of strain S6, strain S6-△U and strain S6＇ was compared and the results suggested that the mutation of URA3 gene would not re-duce the growth of the strain. Accordingly, a method for rapid preparation of auxotrophic brewer＇s strains had been established in this study.