茶树菇活性蛋白组分的体内抗肿瘤活性研究

In vivo Antitumor Activity of the Active Protein Components from Agrocybe aegerita on Tumor-bearing Mouse Model

本文基于大型真菌茶树菇活性抗肿瘤蛋白组分Yt的研究,旨在深入理解其如何通过调节机体免疫系统来发挥生物学功能。通过H22荷瘤小鼠模型,检测瘤内注射Yt(5 mg/kg)抑制肿瘤生长活性,诱导机体产生抗体滴度,并通过再次接种肿瘤细胞检测其是否刺激机体产生免疫记忆功能,最后通过ELISA方法检测血清细胞因子IL-2和TNF-α的表达水平变化。实验发现,瘤内注射Yt可显著抑制小鼠肿瘤生长。Yt、主要蛋白组分Yp和小分子组分Ys均不会诱导机体产生大量抗体而有毒性。Yt治疗后小鼠可获得抗肿瘤免疫记忆功能,且上调血清细胞因子IL-2的表达(84.6±37.6 pg/mL,p<0.05),TNF-α与对照组相比无显著性差异。该数据表明茶树菇活性蛋白组分Yt可激活机体免疫功能,暗示了其作为肿瘤疫苗佐剂的潜力。

The regulation effect of active antitumor protein components Yt from macrofungi Agrocybe aegerita on immune system were investigated in this research. The antitumor activity, the antibody titer, and the immune memory effect were detected on H22-bearing mouse model treated by intratumoral injection of Yt（5 mg/kg）. The alteration expression of serum cytokines IL-2 and TNF-α was also measured by ELISA. The results showed that Yt significantly inhibited the tumor growth. The treatment of Yt, the protein component Yp, and the low weight molecules component Ys did not induce the production of high titer antibody and result in toxicity. Yt induced the long-term memory effect to protect the animals from the tumor cell challenge, up-regulated the expression of serum IL-2（84.6±37.6 pg/mL, p〈0.05）, and did not significantly change the expression of TNF-α. All these data indicated that Yt could active the immune system, and thus can be potentially used as the immune adjuvant of tumor vaccine.