多不饱和脂肪酸对小鼠3T3-L1前脂肪细胞增殖和分化的影响

Effect of n-3/n-6 Polyunsaturated Fatty Acid on the Proliferation and Differentiation of 3T3-L1 Preadipocytes

本文利用MTS比色分析法、细胞形态学分析法及诱导分化法结合油红染色比色分析法研究了α-亚麻酸（alpha-linolenic acid,ALA）、亚油酸（linoleic acid,LA）对3T3-L1前脂肪细胞增殖及分化的影响。MTS结果显示,在一定浓度范围内ALA、LA均能呈剂量依赖关系和时间依赖关系的抑制3T3-L1前脂肪细胞的增殖,且ALA抑制增殖作用强于LA;细胞形态学分析结果显示,ALA、LA在高浓度时均显示出细胞毒性作用;倒置显微镜观察和油红染色检测结果显示,ALA、LA、ALA/LA混合脂肪酸（1：1,n/n）在12.5-200μmol/L浓度范围内均能浓度依赖式的抑制3T3-L1前脂肪细胞分化,且ALA抑制分化作用强于LA,但当浓度〉100μmol/L时,ALA/LA混合脂肪酸（1：1,n/n）的抑制分化作用强于同浓度的ALA,当浓度为200μmol/L时,比较ALA/LA摩尔比为1：4、1：2、1：1的混合脂肪酸抑制分化效果发现,比值为1：1的略强但相互之间效果差异不明显。

The effects of alpha-linolenic acid（ALA） and linoleic acid（LA） on the proliferation and differentiation of 3T3-L1 preadipocytes were investigated by MTS assay, cell morphology analysis and spectrometric method of induction differentiation combined with oil red O staining. MTS results showed that ALA and LA significantly inhibited proliferation of 3T3-L1 preadipocytes in a dose- and time-dependent manner within a certain concentration range, and ALA inhibited proliferation more strongly than LA. Cell morphology analysis showed that ALA and LA were cytotoxic to 3T3-L1 preadipocytes because of high concentration of the fatty acids. The inverted electron microscope observing and oil red staining and detection results showed that ALA, LA and ALA/LA mixed fatty acid（1：1, n/n） significantly inhibited differentiation of 3T3-L1 preadipocytes in a dose-dependent manner in the range of 12.5-200 μmol/L, and ALA inhibited differentiation more efficiently than LA, and the ALA/LA mixed fatty acid（1：1, n/n） showed the strongest inhibition effect on differentiation at the same concentration above 100 μmol/L. When the concentration was 200 μmol/L, the ALA/LA mixed fatty acid（1：1, n/n） showed slightly lower inhibition effect on the differentiation of 3T3-L1 preadipocytes than the ALA/LA mixed fatty acid（1：2, n/n） and the ALA/LA mixed fatty acid（1：4, n/n）.