杂交鲍Haliotis discus hannai×Haliots discus discus体内食源性脂肪酸的标志分异

Differentiation of Food-borne Fatty Acid Biomarkers in Hybrid Abalone Haliotis discus hannai and Haliotis discus discus

本文研究了底播增殖的杂交鲍的内脏团、肌肉和消化腺内的得到公认的几种食源性脂肪酸标志的分布特征,可为杂交鲍的长期食源的判别提供依据。同等大小的杂交鲍,其内脏团、消化腺和肌肉内的硅藻脂肪酸标志(∑C16/∑C18和C20:5n-3)之间无显著无差异;消化腺内的绿藻脂肪酸标志(C18:2n-6+C18:3n-3)显著高于肌肉中的(p<5%);肌肉中的褐藻脂肪酸标志(C20:4n-6)显著低于肌肉内的(p<5%);异养细菌脂肪酸标志(C18:1n-7/C18:1n-9)在同等大小的杂交鲍内脏团、消化腺和肌肉中无差异。内脏团脂肪酸标志受性腺发育的影响;肌肉内脂肪酸含量较低;消化腺中脂肪酸组成的季节变化由食物的季节变化所致且总脂含量较高。从影响脂肪酸组成的因素及测试方法的灵敏度来看,消化腺用于脂肪酸标志法判断杂交鲍的长期食源更适宜。

To determine suitable tissue in hybrid abalone used for long-term food sources, distribution characteristics of food-borne fatty acid biomarkers in digestive gland, muscle and visceral mass of bottom-sowed hybrid abalone were investigated, which were useful to differentiate long-term food sources of hybrid abalone. There were no significant differences in diatom fatty acid biomarkers（∑C16/∑C18 and C20：5n-3） and heterotrophic bacteria fatty acid biomarkers（C18：1n-7/C18：1n-9） among visceral mass, digestive gland and muscle of hybrid abalone with similar body size. The Chlorophyta fatty acid biomarkers（C18：2n-6＋C18：3n-3） in digestive gland was higher than that in muscle（p〈5 %）, while Phaeophyta fatty acid biomarker（C20：4n-6） had the opposite result to Chlorophyta. Fatty acid compositions in visceral mass were affected by the development of gonad; fatty acid content in muscle was low. Total fatty acid content in digestive gland was the highest, and the fatty acid composition varied with the seasonal differentiation of food sources. From points of factors influencing composition of fatty acids and measurement sensitivity, it was better to determine digestive gland as long-term food sources of abalone by fatty acid biomarkers analysis.