菲降解菌株的分离、鉴定及其降解条件的研究

Isolation and Identification of Phenanthrene-degrading Bacteria and Its Degradation Conditions

利用平板升华法从陕北王家川采油厂污染土壤中筛选出3株对菲具有降解活性的菌株F13、F14和FQ20,并对它们降解菲的特性及各种影响因素进行了研究。结果表明,在菲浓度为50 mg/L的条件下,F13在39℃、pH为9、36 h后降解率达到最高值94%;F14在36℃、pH为11、36 h后降解率达到最高值89%;FQ20在36℃、pH为9、30 h后降解率达到最高值87%。根据形态观察和16S rDNA序列分析,鉴定F13、F14、FQ20分别为分支杆菌(Mycobacterium vanbaalenii)、戴尔福特菌(Delftia tsuruhatensis)、敏捷食酸菌(Acidovorax facilis),其16S rDNA序列相似性分别为99.93%、99.27%和99.01%。

Phenanthrene-degrading bacteria F13, F14, FQ20 were isolated from heavy oil contaminated soil at Wangjiachuan oil production factory in the north of Shaanxi province by plating sublimation method. Their abilities for phenanthrene degradation were studied and the various influencing factors were analyzed. The results showed that phenanthrene （50 mg/L） degradation rate for F13 was 94% when pH was 9 after 36 hours rotary culture at 39℃. Phenanthrene （50 mg/L） degradation rate for F14 was 89% when pH was 11 after 36 hours rotary culture at 36℃. Phenanthrene （50 mg/L） degradation rate for FQ20 was 87% when pH was 9 after 30 hours rotary culture at 36 ℃. F13, F14, FQ20 were further identified as Mycobacterium vanbaalenii, Delftia tsuruhatensis and Acidovorax facilis by the 16S rDNA sequencing analysis. The sequence similarities of F13, F14, FQ20 were 99.93%, 99.27% and 99.01%, respectively.