肉桂醛交联壳聚糖包载阿奇霉素微球的制备及表征

Preparation and Characterization of Cinnamaldehyde-crosslinking Azithromycin Drugloaded Chitosan Microsphere

以食品级肉桂醛为交联剂制备阿奇霉素壳聚糖微球,通过正交试验设计法优化,微球制备的最佳工艺为壳聚糖浓度25 mg/L,搅拌速度2 000 r/m,阿奇霉素与壳聚糖质量比1∶2,固化6 h,此时包封率95.1%,载药量20.7%,并对其结构、形态、粒径、包封率、载药量和收率等进行研究,证实壳聚糖的氨基与肉桂醛的醛基发生交联反应;经扫描电镜观察载药微球形貌圆整,表面致密;经差示扫描量热证实药物被包封于壳聚糖微球结构内部,载药性能良好。

Azithromycin-loaded chitosan microsphere was prepared with emulsion crosslinking method and its preparation composition and process was optimized by orthogonality of L9（34）. Chitosan microsphere’s structure, morphology, particle size, entrapment ratio,drug-loaded content,yield were studied. The crosslink reaction between the amino groups on chitosan and the aldehyde on cinnamaldehyde was confirmed in FT-IR spectrum. The good sphericity and dense surface of microsphere were observed under SEM. The drug-loaded properties was good with entrapment efficiency and drug-loaded content of 95.1% and 20.7%, respectively. Cinnamaldehyde as crosslinking agent is suitable for preparing drug-loaded chitosan microsphere.