人类组成型和可变外显子的密码子偏性及聚类分析

Codon Bias and Cluster Analysis of the Constitutive Exons and the Alternative Exons of Human

通过计算人类组成型外显子和可变外显子的RSCU值,对两种外显子的RSCU值进行了比较。结果表明,除了UAG、UGA、UAA 3个终止密码子外,两种外显子的密码子偏性都是相同的。对30条可变外显子和30条组成型外显子序列的RSCU值进行聚类分析,聚类结果不能把两种外显子区别开,证明两种外显子之间的密码子偏性是相同的。对这60条序列的HI进行了聚类分析,聚类结果没有把两种外显子分开。所以外显子序列中没有组成型剪接和可变剪接的信息。

RSCU of the constitutive exons and alternative exons of human were calculated.Two kinds of exons RSCU values were compared.The results showed that every codon bias was the same in two kinds of exons,except three stop codons（UAG,UGA,UAA）.30 constitutive exon sequences and 30 alternative exon sequences were selected to calculate RSCU values of them.The cluster analysis did not distinguish two kinds of exons,proving that the codon biases were the same between the two kinds of exons.HI of the 60 sequences were calculated and used to cluster analysis.It was not able to separate the two kinds of exons.It is indicated that there is no constitutive splicing and alternative splicing information in the exon sequences.