木薯原生质体培养的影响因子研究

Factors affecting Protoplast Culture of Cassava

原生质体技术是细胞工程的核心部分,是进行作物改良和种质创新的重要方法。外植体来源及预处理、酶液组成和浓度、酶解时间、酶液渗透压、培养基组成、培养方法、培养密度等对原生质体培养均有很大影响。对影响木薯(Manihot esculenta Crantz.)原生质体培养再生植株的相关因素进行了综合分析,探讨了原生质体培养在植物育种上的应用潜力。

Protoplast technology,the core of cell engineering,is the important method of crop improvement and germplasm innovation.Source and pretreatment of explant,solution composition and concentration of enzyme,enzymatic duration,osmotic pressure of enzyme solution,composition of the medium,culture methods,culture density and other factors had a great influence on protoplast culture.The factors affecting plant regeneration from protoplast of cassava（Manihot esculenta Crantz.）were analysized comprehensively.The potential of apply protoplast culture in plant breeding were discussed.