摘要
为研究长牡蛎(Crassostrea gigas)免疫基因TLR2-2对MyD88-2基因表达的调控作用,将体外合成的dsRNA注射进入成体长牡蛎体内,72 h后检测TLR2-2基因和MyD88-2基因的表达量。结果表明,成功地对TLR2-2基因和MyD88-2基因进行了干扰,而在TLR2-2基因被干扰后,MyD88-2基因的表达量显著下降,但在MyD88基因被干扰的长牡蛎中TLR2-2基因表达量没有明显变化。
Double strands RNA synthesized in vitro transcription was injected to adult Pacific oysters （Crassostrea gigas）, to study the interaction between TLR2-2 and its potential downstream MyD88 gene. The expression of TLR2-2 and MyD88-2 analyzed by qPCR reduced 72 h after injection. Moreover, in oysters where expression of TLR2-2 was inhibited by dsRNA, MyD88-2 was also found down-regulated, while the expression level of TLR2-2 in oysters where MyD88-2 was suppressed was not significant different compared with that of control group.
出处
《湖北农业科学》
北大核心
2014年第12期2860-2863,共4页
Hubei Agricultural Sciences
