春兰基因组DNA提取方法的比较

Comparative Studies on Extracting Genome DNA of Cymbidium goeringii

为研究了春兰(Cymbidium goeringii)基因组DNA的最佳提取方法,以商洛野生春兰为试验材料,分别采用SDS法、CTAB法、高盐CTAB法提取了春兰基因组DNA。结果表明,高盐CTAB法提取效果较好,其次是CTAB法,SDS法提取效果最差。

To explore the optimal genomic DNA extraction method of Cymbidium goeringii,with wild Cymbidium goeringii in Shangluo as experimental material, genomic DNA of wild Cymbidium goeringii has been extracted by the method of SDS, CTAB and high salt CTAB. The results showed that high salt CTAB was the best of three methods, followed by CTAB and SDS.