一种适于营养胁迫研究的拟南芥水培方法

Simple Hydroponic Method for Nutritional Stress Research of Arabidopsis

水培法可通过更换营养液来控制根际营养成分,成为植物营养学研究的最佳培养方式。由于根际通气和微生物滋生等问题,拟南芥(Arabidopsis thaliana)的水培技术始终无法被广泛应用。该文利用Eppendorf离心管管盖和离心管盒,将拟南芥种子在琼脂上萌发和植株水培有机结合,通过控制营养液用量和液体深度,增加营养液表面积,解决了水培过程中根系通气问题。利用离心管管盖作为支撑材料,降低了琼脂水分蒸发,并减少琼脂的用量和厚度,从而实现琼脂和营养液的快速平衡,为拟南芥培养和营养胁迫研究提供了一个简单且经济的水培方法。

Hydroponics has become the optimal method for plant nutrition research. The technology can control rhizosphere nutrition via a change in nutrient solution. However, hydroponic culture technology in Arabidopsis tha/iana can not be widely used because of root ventilation and microbe creation. In this work, we combined medium germination with hydroponic culture by using Eppendorf tubes and a centrifuge tube box. The problem of root ventilation was resolved by controlling the nutrition culture, reducing culture depth and increasing the surface area. The rapid equilibrium of agar and nutrient solution was achieved by using a centrifugal tube cover as supporting material, which could reduce the evaporation of water and the dosage and thickness of agar. We provide a simple and economical hydroponic method for culture of A. thaliana and nutrition stress research.