ZmLTP3基因提高转基因拟南芥抗盐性的研究

ZmLTP3 Enhances the Salt Resistance in Transgenic Arabidopsis

通过RT-PCR方法从玉米中克隆了一个拟南芥LTP3的同源基因,并命名为ZmLTP3。RT-PCR分析发现,ZmLTP3的表达可以被多种生物和非生物胁迫因子所诱导。通过构建植物表达载体,把ZmLTP3的全长cDNA转化到拟南芥中,以野生型为对照,检测转ZmLTP3基因纯合体株系的抗盐性。结果表明,在盐胁迫条件下,转基因拟南芥生长状况较好,植株鲜重、干重、种子产量以及脯氨酸含量显著高于野生型株系,丙二醛含量和外渗电导率显著低于野生型株系,转基因拟南芥提高了抗盐性。

In previous work, one full-length cDNA encoding an Arabidopsis LTP3 homologue was isolated from maize by RT-PCR and named as ZmLTP3. RT-PCR analysis indicated that the ZmLTP3 expression was induced by many biotic and abiotic stresses. In order to study the biological function of ZmLTP3, the plant expression vector was constructed, and then the full length cDNA of ZmLTP3 was transferred into Arabidopsis. The salt resistance ability of transgenic Arabidopsis homozygotes was checked. The results showed that compared to wild type（WT） lines, transgenic lines grew better, had significantly higher seedling fresh（FW）, dry weight（DW）, seed yields, proline content, meanwhile, had significantly lower MDA content and relative electric conductivity level under salt stress condition.