摘要
目的:建立野葛RAPD分子标记技术并对野葛种质资源遗传背景进行探讨.方法:结合葛根素的测定,应用RAPD技术对11个产地野葛进行遗传多样性分析.结果:葛根素含量在3.26% ~7.10%,8条引物共扩增出45条带,其中37条呈多态性,发现RAPD多态位点为82.22%,证明在野葛种质资源中存在较丰富的遗传多样性.结论:我国野葛具有明显的遗传分化,这些遗传分化是野葛种质资源筛选的关键.
Objective:This study aimed to establish the RAPD molecular marker system and to probe into the genetic background of Pueraria lobata.Method:RAPD analyses of selected plants from eleven various habitats in China were carried out using arbitrary primers,in combination with the chemical investigation of puerarin.Result:The percentage of puerarin ranged from 3.26% to 7.10%.A total of 45 DNA fragments were obtained using 8 primers,37 of which were polymorphic,accounting for 82.22%,which can tell that the samples had a high level of genetic diversity.Conclusion:P.lobata has significant genetic differentiation that further support the prospects for germplasm selection.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第16期56-59,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技基础条件平台项目(2005DKA21000)
河南省教育厅科学技术研究重点项目(13A360557)
关键词
野葛
葛根素
遗传多样性
随机扩增多态性DNA
Pueraria lobata
puerarin
genetic diversity
random amplified polymorphic DNA