野葛种质资源的随机扩增多态性DNA技术分析

RAPD Analysis of Germplasm Resource in Pueraria lobata

目的：建立野葛RAPD分子标记技术并对野葛种质资源遗传背景进行探讨.方法：结合葛根素的测定,应用RAPD技术对11个产地野葛进行遗传多样性分析.结果：葛根素含量在3.26％ ～7.10％,8条引物共扩增出45条带,其中37条呈多态性,发现RAPD多态位点为82.22％,证明在野葛种质资源中存在较丰富的遗传多样性.结论：我国野葛具有明显的遗传分化,这些遗传分化是野葛种质资源筛选的关键.

Objective：This study aimed to establish the RAPD molecular marker system and to probe into the genetic background of Pueraria lobata.Method：RAPD analyses of selected plants from eleven various habitats in China were carried out using arbitrary primers,in combination with the chemical investigation of puerarin.Result：The percentage of puerarin ranged from 3.26％ to 7.10％.A total of 45 DNA fragments were obtained using 8 primers,37 of which were polymorphic,accounting for 82.22％,which can tell that the samples had a high level of genetic diversity.Conclusion：P.lobata has significant genetic differentiation that further support the prospects for germplasm selection.