蜘蛛香总环烯醚萜中chlorovaltrate和蜘蛛香素B的测定

Content Determination of Chlorovaltrate and Valjatrate B in Total Iridoids of Valerianae Jatamansi Rhizoma et Radix

目的：运用高效液相色谱法测定蜘蛛香总环烯醚萜中chlorovaltrate和蜘蛛香素B的含量.方法：采用高效液相色谱法,waters C18色谱柱（4.6 mm×250 mm,5μm）;chlorovaltrate和蜘蛛香素B的流动相分别为乙腈-水（60∶40）,（50：50）;检测波长分别为254,203 nm;流速均为1.0 mL·min-1;柱温均为30℃.结果：蜘蛛香总环烯醚萜中chlorovaltrate和蜘蛛香素B进样量分别在15.5 ～155,20 ～200 pg与各自峰面积积分值呈良好线性关系（r分别为0.998 6,0.998 7）;测得chlorovaltrate和蜘蛛香素B含量分别为8.91,7.00 mg·g-1.结论：确定了蜘蛛香总环烯醚萜中chlorovaltrate和蜘蛛香素B的含量,为蜘蛛香总环烯醚萜的质量控制提供了一定依据.

Objective：The purpose of this study was to determine the content of chlorovaltrate and valjatrate B in total Iridoids of Valerianae Jatamansi Rhizoma et Radix.Method：HPLC method was adopted.The chromatographic column was waters C1s column （4.6 mm ×250 mm,5 μm） ; the mobile phase for chlorovaltrate and valjatrate B was acetonitrile water （60∶40）,（50∶ 40）; the detection wavelength for chlorovaltrate and valjatrate B were 254,203 nm; the flow rate was 1.0 mL ·min-1 ; the column temperature was 30 ℃.Result：The fine linear correlation displayed when the injection volumn were 15.5-155 pg （r =0.998 6）,20-200 pg （r =0.998 7） for chlorovaltrate and valjatrate B in total iridoids of Valerianae Jatamansi Rhizoma et Radix,respectively.The contents of chlorovaltrate and valjatrate B were 8.91 mg ·g-1 and 7.00 mg ·g-1,respectively.Conclusion：The contents of chlorovaltrate and valjatrate B in total Iridoid of Valerianae Jatamansi Rhizoma et Radix were determined to provide evidence for quality control of total iridoids of Valerianae Jatamansi Rhizoma et Radix.