HPLC测定艾蒿黄酮乳膏中槲皮素和柚皮素的含量

HPLC Determination of Quercetin and Naringenin in Moxa Flavone Cream

目的：建立以高效液相色谱法测定艾蒿黄酮乳膏中槲皮素和柚皮素含量的方法.方法：Gemini C18反相色谱柱（4.6mm×250mm,5μm）,流速1.0 mL·min-1,柱温25℃,检测波长330 nm,进样量10 μL,流动相0.5％乙酸水溶液（A）-乙腈（B）梯度洗脱.结果：槲皮素在0.010 26 ～0.307 8 μg（r=0.999 9）线性关系良好,平均回收率为97.32％,RSD 3.3％;柚皮素在0.009 87 ～0.296 1 μg（r=1）线性关系良好,平均回收率为97.22％,RSD 2.7％.结论：该方法简便、快速、重复性好,可用于艾蒿黄酮乳膏的质量控制.

Objective：The study was conducted to establish an HPLC method for the determination of quercetin and naringenin in the moxa flavone cream.Method：Quercetin and naringenin were separated on Gemini C18 RP-column （4.6 mm × 250 mm,5 μm）,the flow rate was 1.0 mL ·min-1 column temperature was 25 ℃ and the detection wavelength was 330 nm,the injection volume was 10 μL,with 0.5％ aqueous acetic acid as the mobile phase A was and acetonitrile as B for gradient elution.Result：Quercetin and naringenin showed good linear relationship in the range of 0.010 26 to 0.307 8 μg （r=0.999 9）,0.009 87 to0.296 1 μg （r =1） respectively.Their average recovery rates were 97.32％ with RSD of 3.3％ and 97.22％ with RSD of 2.7％,respectively.Conclusion：The method is simple,rapid,reproducible,and it can be used for the quality of control moxa flavone cream.