附子水溶性生物碱对心衰细胞模型的治疗作用

Therapeutic Effects of Water-solubility Alkaloid of Acoitum carmichaelii on Heart-failure Cell Model

目的:研究附子水溶性生物碱对心力衰竭大鼠心肌细胞膜ATP酶以及相关离子的影响,以揭示其对急性心力衰竭细胞的治疗作用。方法:取新生大鼠心室肌细胞原代培养5 d至细胞成熟,用0.8%戊巴比妥钠作用5 min造模后,分别给予附子水溶性生物碱0.01,0.02,0.04 g·L-1,以及阳性药物去乙酰毛花苷注射液4 mL·L-1,作用1.5 h后检测心肌细胞ATP酶活性以及各相关离子浓度。结果:模型组与正常组比较,心肌细胞活力明显减小,细胞内Ca2+-ATP酶和Ca2+-Mg2+-ATP酶的活性与Na+,Mg2+含量降低,K+,Ca2+的含量与Na+-K+-ATP酶的活性上升;去乙酰毛花苷4 mL·L-1以及附子水溶性生物碱各剂量组均能提高心衰细胞的细胞活性,并能升高模型细胞Na+,Mg2+含量,提高细胞内Ca2+-ATP酶和Ca2+-Mg2+-ATP酶的活性,降低K+,Ca2+的含量与Na+-K+-ATP酶的活性。结论:附子水溶性生物碱能调节心衰细胞内酶的活力与离子浓度使之趋于正常,对戊巴比妥钠致心衰细胞模型具有一定的治疗作用。

Objective：To reveal the therapeutic effects of water-solubility alkaloid after using of Aconitum carmichaelii on heart-failure cell model by studying its influence on myocardial cells＇ membrane ATP enzyme and relative ions.Method：Culture the myocardial cells,gotten from the neonatal rats,for 5 days until the cells became mature,then nembutal （0.8％） was used to establish the heart-failure cell model for 5 min administrate them with 3 concentrations of A.carmichaelii water-solubility alkaloid （0.01,0.02,0.04 g ·L-1） anddeslanoside injection （4 mL · L-1） determine the vitality of the myocardial cells＇ membrane ATP enzyme and theconcentration of relative ions 90 min later.Result：Compared with the blank control group,in the model group the survival rates for cells were restrained,the concentrate of Na ＋,Mg2＋ and the activity of Ca2＋-ATP enzyme,Ca2＋-Mg2＋-ATP enzyme was decreased.On the contrary,the concentrate of K ＋,Ca2＋ and the activity of Na ＋-K ＋-ATP enzyme was increased.Deslanoside injection （4 mL · L-1） and A.carmichaelii water-solubility alkaloid （1.25 × 10-3,2.50 × 10-3,5.00 × 10-3g · kg-1） could increase the the survival rates of heart-failure cells and the concentrate of Na＋,Mg2＋,and also enhance the activity of Ca2＋-ATP enzyme,Ca2＋-Mg2＋-ATP enzyme.Conversely,all the administrated groups decreased the concentrate of K ＋,Ca2＋ in the myocardial cells and inhibited the activity of Na＋-K＋-ATP enzyme.Conclusion：Water-solubility alkaloid of A.carmichaelii has the therapeutic effect on heart-failure cells.