摘要
通过物理交联和化学交联并用的方式制备胶原基复合水凝胶。通过红外光谱(FT—IR)、差示量热扫描(DSC)、x射线衍射分析(XRD)、扫描电子显微镜(SEM)观察等手段,对胶原基复合水凝胶的基团组成、结晶度、结构及形貌等情况进行分析。FT-IR结果表明:水凝胶中的基团组成符合原料组成的特点,能够定性判断聚乙烯醇与胶原形成共价交联。DSC法计算的水凝胶中结晶度为27.5%左右。XRD分析中出现了聚乙烯醇的特征吸收峰,验证其晶体结构的存在。SEM结果显示胶原基复合水凝胶具有多孔结构。同时,水凝胶的溶胀特性、透水汽性良好。细胞毒性试验表明,胶原的存在能够促进成纤维细胞的生长,细胞毒性等级为0~1级,符合医用创面敷料的基本要求。
The polyvinyl alcohol - collagen hydrogels was prepared by the method of physical crosslinking and chemical crosslinking. The composition groups, crystallinity, structure and surface morphology of compound hydrogels were charactered by FT - IR, DSC, XRD and SEM et al. The FT - IR results indicate that the composition groups of compound hydrogels are in accordance with the composition of raw materials, and it forms covalent cross - linking between PVA and collagen. DSC results show that the erystallinity of compound hydrogels is about 27.5 %. XRD results indicate that the appearance of characteristic absorption peak of PVA confirms the existence of crystal structure. SEM results show that the compound hydrogels have a porous structure, good swelling properties and water vapor permeability. The eytotoxicity experiment reveals that the presence of collagen can promote the growth of fibrocyte. And the cytotoxicity grade is 0 - 1 ,which meet the standards of biological wound dressing.
出处
《中国皮革》
CAS
北大核心
2014年第15期16-19,27,共5页
China Leather
基金
国家自然科学基金项目(No:21276164)
国家科技支撑计划项目(No:2011BAC06B11)
关键词
胶原
聚乙烯醇
水凝胶
生物学性能
collagen
polyvinyl alcohol
hydrogel
biological property