Toll样受体4与鼠颞下颌关节滑膜炎的相关性研究

Studies on the correlation between the expression of Toll-like receptor 4 and the synovitis of the temporomandibular joint in rats

目的 探讨Toll样受体4（Toll-like receptor-4,TLR-4）与大鼠颞下颌关节（temporomandibular joint,TMJ）滑膜炎的相关性.方法 选取60只雄性Wistar大鼠,通过随机数字表随机分为5组,每组12只.A组（空白对照组）：正常饮食,不做任何处理;B组（咬肌切除组）：切除大鼠双侧咬肌;C组（咬合干扰组）：右下第一磨牙粘接铸造金属冠;D组（咬合升高组）：粘接上颌磨牙（牙合）垫;E组（咬肌切除加咬合升高组）：切除双侧咬肌并粘接上颌磨牙（牙合）垫.取右侧TMJ作为样本,HE染色观察滑膜的变化并进行病理学评分,免疫组化染色检测TLR-4蛋白表达,实时定量PCR检测TLR-4mRNA表达,分别对TLR-4蛋白及mRNA表达与病理学评分进行Spearman相关性分析.结果 A～E组的病理学评分分别为0.5 ±0.5、2.5±1.0、2.7± 1.0、3.0± 0.9、5.3± 1.2,TLR-4蛋白表达分别为（3.2±1.5）％、（16.0±2.6）％、（15.8±2.1）％、（17.5±2.4）％、（38.2±4.4）％,TLR-4 mRNA表达分别为1.07±0.09、2.12±0.33、2.07±0.29、2.17±0.34、4.53±0.46.与A组相比,B～E组病理学评分、TLR-4蛋白和TLR-4 mRNA表达均显著升高（P＜0.05）,TLR-4蛋白与病理学评分显著相关（r=0.785,P＜0.05）,TLR-4mRNA表达与病理学评分显著相关（r=0.720,P＜0.05）.结论 TLR-4可能参与了大鼠TMJ滑膜炎的发病过程.

Objective To investigate the expression of the Toll-like receptor-4 （TLR-4） in temporomandibular joint synovitis in rats,and to discuss the correlation between the expression of TLR-4 and the synovitis.Methods Sixty male wistar rats were randomly divided into five groups,12 each.Group A was the control group in which the rats were given normal diet.In Group B,the rats＆#39; bilateral masseter muscles were cut off （masseter resection group）.In Group C,An cast metal crown were bonded on the mandibular right first molar of each rat （occlusal interference group）.In Group D,occlusal pad were bonded on maxillary molars of each rat （occlusal dimension increase group）.In Group E,rats＆#39; bilateral masseter muscles were resected and occlusal pads were bonded on their maxillary molars （masseter resection and occlusal dimension increase group）.Pathological changes of synovium were observed using hematoxylin and eosin （HE） stains and pathology scores were evaluated.The expression of TLR-4 were determined by immunohistochemical stains,and the expression of TLR-4 mRNA were determined by real-time PCR.The correlation between the expression of TLR-4,TLR-4 mRNA and the pathological score were analyzed using Spearman analysis.Results The pathological scores of Group A-E were 0.5±0.5,2.5±1.0,2.7±1.0,3.0±0.9,5.3±1.2 respectively.The expression of TLR-4 were （3.2±1.5） %,（16.0±2.6） %,（15.8±2.1） %,（17.5±2.4） %,（38.2±4.4） %.The expression of TLR-4 mRNA were 1.07±0.09,2.12±0.33,2.07±0.29,2.17±0.34,4.53±0.46.Compared with group A,groups B-E showed significant higher pathology score （P〈0.05） and increased expression of both TLR-4 （P〈0.05） and TLR-4 mRNA （P〈0.05）.An significant positive correlation was found between the expression of TLR-4 and the pathology score （r=0.785,P〈0.05）,and between the expression of TLR-4 mRNA and the pathology score （r=0.720,P〈0.05）.Conclusions TLR-4 may be closely associated with the development of the synovitis of TMJ of rats.