比色法测定不同产地马齿苋中总多酚

Determination of total polyphenols in Portulaca oleracea from various habitats by colorimetric method

目的：建立马齿苋药材中总多酚的测定方法，考察不同产地马齿苋药材中总多酚，为马齿苋药材质量评价提供参考。方法以没食子酸为对照品，采用福林酚（FC）比色法测定马齿苋中总多酚。结果不同产地的马齿苋药材中多酚含量在6.30-17.37 mg/g。结论 FC比色法简单快速，稳定，结果准确，适用于马齿苋中总多酚的测定；不同产地马齿苋中总多酚差异较大，建议马齿苋药材中总多酚质量分数以没食子酸计不低于6 mg/g。

Objective To establish a method for determination of total polyphenols inPortulaca oleracea, to investigate the total polyphenols in this herb from various habitats, and to provide reference for quality evaluation ofP.oleracea.Methods The total polyphenols content in P.oleracea was determined by Folin-Ciocalteu （FC） colorimetric method with gallic acid as the standard. Results The total polyphenols of 11 batches ofP.oleracea varied from 6.30 to 17.37 mg/g.Conclusion FC colorimetric method is a quick, easy-operating, precise, and stable method, which is suitable for the determination of total polyphenols inP.oleracea. In consideration of big differences of total polyphenols amongP.oleracea from various habitats, it is suggested that the total polyphenols inP.oleracea crude drug should be above 6 mg/g.