PIMT对类风湿关节炎成纤维样滑膜细胞凋亡的影响

Influence of PIMT on apoptosis of fibroblast-like synoviocytes in rheumatoid arthritis

目的:探讨蛋白异天冬氨酰甲基转移酶(Protein isoaspartyl-methyltransferase,PIMT)过表达对类风湿关节炎(Rheumatoid arthritis,RA)成纤维样滑膜细胞(Fibroblast-like synoviocytes,FLS)凋亡的影响。方法:构建PIMT表达载体,转染RA-FLS细胞,检测PIMT在RA-FLS细胞表达水平,观察转染后RA-FLS细胞的凋亡情况。结果:转染后的RA-FLS细胞PIMT mRNA和蛋白质表达水平上升,与正常对照和转染空载体的RA-FLS细胞阴性对照相比较,转然后RA-FLS细胞的凋亡增加。结论:PIMT在RA-FLS细胞中表达下降是RA-FLS细胞凋亡减少的一个重要原因,PIMT在RA-FLS细胞增殖/凋亡失调中起作用。

To prove the influence of protein isoaspartyl-methyltransferase （ PIMT ） on the cell apoptosis of fibroblast-like synoviocytes of RA.Methods： The expression vector of PIMT was constructed and transfected in to the RA-FLS, the impact of PIMT on the cell apoptosis of RA-FLS was observed by overexpressing the vector of PIMT.Results：The mRNA and protein level of PIMT in RA-FLS was increased after transfected the vector of PIMT into RA-FLS;compared with the normal cultured RA-FLS and the RA-FLS transfected with the empty vectors ,the cell apoptosis level was also increased.Conclusion：The decreased expression level of PIMT in RA-FLS is an important reason for reduce apoptosis of RA-FLS,and PIMT can affect the imbalance of proliferation/ap-optosis in the RA-FLS.