cGVHD患者外周血中CD45RA^+和CD45RO^+ T淋巴细胞亚群的特点及其临床意义

Characteristics of peripheral blood CD45RA^+and CD45RO^+ T lymphocyte subsets in patients with cGVHD and its clinical significance

目的:探讨同种异基因造血干细胞移植(allo-HSCT)后发生慢性移植物抗宿主病(cGVHD)患者外周血中CD45RA+和CD45RO+T淋巴细胞亚群特点及其与cGVHD发生之间的关系。方法:回顾性研究广东省人民医院2001年3月~2009年9月64例造血干细胞移植植入成功的患者,其中无cGVHD发生21例,轻度cGVHD 15例,中度cGVHD 18例,重度cGVHD 10例,应用流式细胞术,对其外周血中CD4+CD45RA+、CD4+CD45RO+、CD8+CD45RA+和CD8+CD45RO+T淋巴细胞亚群进行检测。结果:与无cGVHD患者相比,cGVHD患者CD4+CD45RA+T细胞百分比均明显降低(P<0.05),CD4+CD45RO+T细胞百分比均明显增高(P<0.05),而CD8+CD45RA+、CD8+CD45RO+T细胞百分比均无明显改变。轻、中、重度的cGVHD患者之间比较,CD4+CD45RA+、CD4+CD45RO+、CD8+CD45RA+和CD8+CD45RO+T细胞百分比均无明显改变。结论:CD4+CD45RA+和CD4+CD45RO+T细胞可能对cGVHD的发生有重要意义。

To characterize the CD45RA＋and CD45RO＋T lymphocyte subsets in the peripheral blood of patients with cGVHD induced by allogeneic haematopoietic stem cell transplantation （ allo-HSCT ） and to explore their relations with the disease.Methods：The peripheral blood was collected from 64 patients after allo-HSCT,including 21 non-cGVHD patients,15 light grade cGVHD patients,18 mild grade cGVHD patients and 10 severe grade cGVHD patients,then CD4＋CD45RA＋,CD4＋CD45RO＋, CD8＋CD45RA＋and CD8＋CD45RO＋T lymphocyte subsets were detected by flow cytometry （ FCM）.Results： Compared with the control,the percent of CD4＋CD45RA＋T lymphocyte in patients with light,mild and severe grade cGVHD decreased markedly （P〈0.05）,the percent of CD4＋CD45RO＋T lymphocyte increased markedly （P〈0.05）.But there were not obviously change in the patient with different grade cGVHD.The percent of CD8＋CD45RA＋,CD8＋CD45RO＋T lymphocyte did not change obviously.Conclusion：CD4＋CD45RA＋and CD4＋CD45RO＋may play an important role in the pathogenesis of cGVHD.