高血压大鼠穹窿下器神经元型一氧化氮合酶阳性神经元的表达及意义

The expression and significance of nitric oxide synthase positive neurons in subfornical organ of hypertensive rats

目的观察高血压大鼠穹窿下器内神经元型一氧化氮合(nNOS)酶阳性神经元的表达及意义。方法雌性Wistar大鼠予以左旋硝基精氨酸(L-NNA)15 mg·d-1·kg-1腹腔注射,成功制备高血压动物模型。随机选择对照组、用药2周组、用药4周组、用药8周组,常规经心灌注固定取脑,取材后经处理进行nNOS免疫显色观察。结果nNOS免疫阳性细胞染色随着血压的升高呈现先加深后变浅的趋势。用药组与对照组比较差异有统计学意义(P<0.05)。结论穹窿下器nNOS免疫阳性神经元的改变有可能是通过影响血压调节中枢的交感活性而间接调控高血压的发生,和高血压的变化有关。

Objective To observe the expression and significance of nitric oxide synthase positive neurons in subfornical organ （SFO） of hypertensive rats. Methods The female Wistar rats were injected with L-nitro-arginine （L-NNA） 15 mg· d-1· kg-1intraperitoneally to establish animal models with hypertension. These rats were randomly were divided into control group,2 -week medication group, 4-week medication group, 8-week medication group, the rats in each group were treated by conventional cardiac perfusion and fixation, then the rats＇ brains were taken out and the paraffin sections were stained with nNOS immunohistochemical staining. Results The immunopositive cells of nNOS within SFO were stained at first deeply, then became shallower with the increase of blood pressure. Conclusion The changes of neuronal nitric oxide synthase immunoreactive neurons in subfornical organ of hypertensive rats may be related with the changes of hypertension, which may be caused by affecting consensus activity in blood pressure regulation center to regulate indirectly the occurrence of hypertension.