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瑞舒伐他汀对大鼠心肌缺血再灌注后细胞凋亡及线粒体融合素2表达的影响 被引量：4

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摘要 目的探讨瑞舒伐他汀对大鼠心肌缺血再灌注损伤后细胞凋亡及线粒体融合素2蛋白表达的影响。方法选取雄性SD大鼠32只,随机分为假手术(Sham)组、缺血再灌注(I/R)组、瑞舒伐他汀1(Statin 1)组和瑞舒伐他汀2(Statin2)组,每组8只。Statin1组术前7天开始给予瑞舒伐他汀5 mg/(kg·d)灌胃,Statin2组药物剂量为20 mg/(kg·d),I/R组以蒸馏水灌胃,随后制备心肌缺血再灌注损伤模型。各组于再灌注3 h后剪取心脏缺血再灌注损伤区域,脱氧核苷酸末端转移酶介导的缺口末端标记法检测细胞凋亡,免疫印迹法检测磷酸化蛋白激酶B(p-Akt)的表达,免疫组化法检测线粒体融合素2的表达。结果与Sham组相比,I/R组及Statin 1、2组心肌细胞凋亡显著增加,p-Akt表达显著下降,线粒体融合素2表达显著增加(P<0.01);与I/R组相比,Statin 1、2组心肌细胞凋亡和线粒体融合素2表达均显著降低(P<0.05),而p-Akt表达显著增高(P<0.05),且Statin 2组较Statin 1组变化更显著(P<0.05)。结论瑞舒伐他汀可抑制大鼠心肌缺血再灌注损伤后的细胞凋亡,其作用可能与抑制线粒体融合素2表达有关。
作者 周炜 陈玲 陈曼华
机构地区 湖北省武汉市中心医院心内科
出处 《广东医学》 CAS CSCD 北大核心 2014年第16期2514-2516,共3页 Guangdong Medical Journal
基金 国家自然科学基金资助项目(编号:81300214) 湖北省自然科学基金资助项目(编号:2013CFB378)
关键词 瑞舒伐他汀 缺血再灌注 心肌细胞 凋亡 线粒体融合素2基因
分类号 R542.2 [医药卫生—心血管疾病]
  • 相关文献

参考文献14

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共引文献5

同被引文献39

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引证文献4

二级引证文献23

广东医学
2014年 第16期

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