结直肠腺癌中特异蛋白1表达与临床病理指标的关系

Relationship between expression of specificity protein 1 of colorectal adenocarcinoma and clinicopathological parameters

目的研究结直肠腺癌组织中特异蛋白1(Sp1)表达的变化及其与患者临床病理学指标的关系。方法通过查阅mRNA array数据库Oncomine,分析结直肠腺癌组织及正常组织中Sp1 mRNA的水平和差异。采用免疫组织化学法检测65例结直肠腺癌组织及其癌旁正常组织中Sp1蛋白的表达,Western blotting检测5个不同恶性程度的结直肠癌细胞系中Sp1蛋白的表达,分析Sp1表达水平与结直肠癌患者临床病理学指标之间的关系。结果查阅Oncomine数据库发现Sp1 mRNA在结直肠腺癌组织中的表达与正常组织无明显差异。免疫组织化学法及细胞系Western blotting检测结果证实,Sp1在结直肠腺癌组织和细胞系中均有较高的一致性表达,但与癌旁正常组织无差异;肿瘤组织中Sp1蛋白表达水平与结直肠腺癌患者淋巴结和远处转移与否无明显相关性。结论转录因子Sp1在结直肠腺癌组织中的表达与正常组织无明显差异,与肿瘤转移无明显相关性。

Objective To investigate the expression changes of specificity protein 1 （Spl） in colorectal adenocarcinoma tissues and its relationship with the clinicopathological parameters of patients. Methods The levels and differences of Spl mRNA of colorectal adenocarcinoma tissues and normal tissues were analyzed by retrieving the mRNA array database of Oncomine. The expression of Spl protein of 65 colorectal adenocarcinoma tissues and adjacent normal tissues was detected by the immunohistochemistry （IHC） assay. The expression of Spl protein of colorectal cancer ceil lines of 5 different malignant degrees was detected by the Western blotting. The relationship of the expression level of Sp1 and the clinicopathological parameters of patients with colorectal adenocarcinoma was analyzed. Results There was no significant difference of Sp1 mRNA level between colorectal adenocarcinoma tissues and normal tissues according to the mRNA array database of Oncomine. The results of immunohistochemistry and Western blotting showed that the expressions of Sp1 of colorectal adenocarcinoma tissues and cell lines were highly consistent, but were not different from those of adjacent normal tissues. There was no significant correlation between the expression level of Sp1 protein of colorectal adenocarcinoma tissues and lymph node metastasis, as well as distant metastasis. Conclusion The expression of Sp1 of colorectal adenocarcinoma tissues is not significantly different from that of normal tissues and is not significandy correlated to the cancer metastasis.