青春前期十溴联苯醚暴露对雄性大鼠精子质量的影响

Effects of BDE-209 exposure on sperm quality of prepubertal male rats

目的探讨青春前期十溴联苯醚(BDE-209)暴露对雄性大鼠精子质量的影响。方法将24只3周龄雄性SD大鼠随机分为对照组和染毒组。于出生后第28日开始,染毒组分别给予2、10和50 mg/kg BDE-209(低、中、高剂量染毒组)灌胃染毒1个月,对照组给予纯玉米油灌胃1个月,于出生后第63日处死大鼠,观察精子形态学变化,计算精子畸形率,测定精子运动参数。结果在染毒组可观察到香蕉形、无钩、胖头等典型的精子形态学改变。低、中、高剂量染毒组精子畸形率分别为(10.50±3.987)‰、(11.50±6.775)‰和(10.83±4.401)‰,对照组精子畸形率为(4.00±3.688)‰,各染毒组精子畸形率均显著高于对照组,差异有统计学意义(P<0.05)。低、中、高剂量染毒组精子运动参数与对照组比较,差异均无统计学意义(P>0.05)。结论 BDE-209暴露对雄性SD大鼠的精子质量有一定的影响。

Objective To investigate the effects of BDE-209 exposure on the sperm quality of prepubertal male rats. Methods Twenty four 3-week old SD rats were randomly divided into the control group and three BDE- 209 groups. BDE-209 groups were given BDE-209 of 2, 10, and 50 mg/kg by the intragastric injection for one month after 28 d of birth. The control group was given pure corn oil by the intragastric injection for one month. Rats were sacrificed on Day 63. The morphological changes of sperms were observed. The deformity rate of sperms was calculated and kinesis parameters were measured. Results Typical morphological changes such as banana-shaped, no hook, and fat head were observed in BDE-209 groups. The deformity rates of BDE-209 groups were （10.50 ±3. 987） ‰, （11.50 ±6. 775） ‰, and （10.83 ±4. 401） ‰, respectively. The deformity rate of control group was （4. 00±3. 688）. The deformity rates of BDE-209 groups were significantly higher than that of the control group （P〈0.05）. The differences of kinesis parameters of BDE-209 groups and the control group were not statistically significant （P〉0. 05）. Conclusion Exposure to BDE-209 has certain influence on the sperm quality of male SD rats.