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同源重组方法在制备DNA大片段中的应用 被引量：5

Manipulation of large DNA fragments using homologous recombination
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摘要 目的介绍快速、简便制备DNA大片段的新方法。方法查阅国内外近年关于DNA大片段制备方法的文献33篇,对这些方法进行归纳和总结。结果综述了大肠杆菌Red/ET重组系统和基于酿酒酵母重组系统的DNA自组装和TAR克隆在DNA片段制备中的应用。结论同源重组系统不仅提高了克隆DNA大片段的效率,而且简化了操作流程。这些方法在制备DNA大片段用于组合生物合成和合成生物学制备药物中具有重要的应用前景。 Objective To introduce efficient and simple methods used for preparing large DNA fragments. Methods The preparation methods of large DNA fragments were analyzed and summarized based on pub- lished articles. Results The application of Red/ET of E. coli, DNA assembler and transformation-associated recombination（TAR） of Saccharomyces cerevisiae were reviewed. Conclusions Clone DNA based on ho- mologous recombination not only improve large DNA clone efficiency, but also simplify the procedures. Those methods have broad application prospects in the preparation of large DNA fragments, which is used for the manufacture of biological medicines by combinatorial biosynthesis and synthetic biology.
作者 倪现朴 季州翔 夏焕章
机构地区 沈阳药科大学
出处 《沈阳药科大学学报》 CAS CSCD 北大核心 2014年第8期663-668,共6页 Journal of Shenyang Pharmaceutical University
基金 国家自然科学基金资助项目(81273410) 辽宁省博士科研启动基金资助项目(20121103)
关键词 合成生物学 同源重组 RecET系统 DNA自组装 酵母TAR克隆 synthetic biology homologous recombination RecET DNA assembly transformation-associ-ated recombination （TAR）
分类号 S859.797 [农业科学—临床兽医学]
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参考文献33

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同被引文献73

  • 1陈向岭,袁汉英,何炜,胡翔华,吕红,李育阳.通过同源重组构建酿酒酵母新型表达质粒[J].中国科学（C辑）,2005,35(1):37-43. 被引量：3
  • 2陈悦康,李活,王飞,聂冬梅,尚桂芳.核酸筛查实验室RNA酶污染防止对策探讨[J].中国自然医学杂志,2007,9(2):137-138. 被引量：11
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  • 4Sleight SC, Bartley BA, Lieviant JA, et al. In-Fusion BioBrick assembly and re-engineering[J]. Nucleic Acids Research, 2010, 38(8): 2624-2636.
  • 5Anderson JC, Dueber JE, Leguia M, et al. BglBricks: a flexible standard for biological part assembly[J]. Journal of Biological Chemistry, 2014, 4(1): I.
  • 6Vick JE, Johnson ET, Choudhary S, et al. Optimized compatible set of BioBrick? vectors for metabolic pathway engineering[J]. Applied Microbiology and Biotechnology, 2011, 92(6): 1275-1286.
  • 7Chaudhary AK, Park JW, Yoon YJ, et al. Re-engineering of genetic circuit for 2-deoxystreptamine (2-DOS) biosynthesis in Escherichia coli BL2I(DE3)[J]. Biotechnology Letters, 2013, 35(2): 285-293.
  • 8Guo JH, Huang FL, Huang C, et al. Specificity and promiscuity at the branch point in gentamicin biosynthesis[J]. Chemistry & Biology, 2014, 21(5): 608-618.
  • 9Engler C, Gruetzner R, Kandzia R, et al. Golden gate shuffling: a one-pot DNA shuffling method based on type lIs restriction enzymes[J]. PLoS One, 2009, 4(5): e5553.
  • 10Kodumal SJ, Patel KG, Reid R, et al. Total synthesis of long DNA sequences: Synthesis of a contiguous 32-kb polyketide synthase gene cluster[J]. Proceedings of the National Academy of Sciences of the United States of America, 2004, 101(44): 15573-15578.

引证文献5

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沈阳药科大学学报
2014年 第8期

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