摘要
目的:建立HPLC法测定牛黄上清胶囊中栀子苷的含量.方法:采用Agela C1s色谱柱(250 mm×4.6 mm,5μm),以甲醇-水(20∶ 80)为流动相,流速为0.8 ml/min,检测波长为238 nm.结果:栀子苷线性范围为0.101 7 ~0.6102μg/ml(r=0.999 9),平均回收率为99.53%,RSD为0.57%(n=6).结论:本方法可靠、灵敏、准确,适用于牛黄上清胶囊中栀子苷的质量控制.
Objective: To establish a HPLC method for determinative of geniposide in Niuhuangshangqing Capsules. Method: The Agela C18 column (250 mm×4.6 mm,5μm) was used with the mobile phase consisted of methanol -water(20: 80) ,the flow rate was 0.8 ml/min, the detection wavelength was set at 238 nm. Result: The linear range of geniposide was 0. 101 7-0. 610 2 μg/ml(r =0.999 9) ,the average recovery rate was 99.53% ,RSD was 0157% (n =6). Conclusion: The method is relia- ble,sensitive,accurate and can be used for the quality control of Niuhuangshangqing Capsules.
出处
《天津药学》
2014年第4期18-19,72,共3页
Tianjin Pharmacy