NOD2基因对舌鳞癌Tca8113细胞增殖和凋亡的影响

The effects of NOD2 gene on the proliferation and apoptosis of human tongue squamous cell carcinoma Tca8113 cells

目的:观察NOD2基因对舌鳞癌Tca8113细胞增殖、凋亡的影响。方法:构建NOD2表达载体(NOD2-pEZ-M29)和NOD2-shRNA表达载体,分别转染舌鳞癌Tca8113细胞48 h后,RT-PCR和Western blot法检测NOD2和HBD-2分子及蛋白表达,MTT法检测细胞增殖,流式细胞仪检测细胞凋亡比例。结果:转染NOD2表达载体的Tca8113细胞NOD2和HBD-2表达较对照组显著增高,细胞增殖速度较对照组慢,凋亡较对照组高;转染NOD2-shRNA载体的Tca8113细胞NOD2和HBD-2表达较对照组显著降低,细胞增殖速度较对照组快,凋亡较对照组低。结论:NOD2可促进舌鳞癌细胞凋亡,抑制其增殖。在舌鳞癌细胞中,NOD2与HBD-2的表达呈正相关。

Objective：To explore the effects of NOD2 gene on the proliferation and apoptosis of tongue squamous cell carcinoma Tca8113 cells.Methods：NOD2 expression vector（NOD2-pEZ-M29）and NOD2-shRNA vector were established,then were transfected into Tca8113 cells respectively.Expressions of HBD-2 and NOD2 in the cells was detected by RT-PCR and Western blot.Cell proliferation was examined by MTT assay and apoptosis by flow cytometry at 48h post transfection.Results：Compared with the control group,the expression of NOD2 and HBD-2 in NOD2-pEZ-M29 transfection group was significantly higher and markedly lower in NOD2-shRNA group.The proliferation rate of Tca8113 cells was markedly lower in NOD2-pEZ-M29 transfection group and significantly higher in NOD2-shRNA group while the apoptosis rate was significantly higher in NOD2-pEZ-M29 transfection group and significantly lower in NOD2-shRNA group.Conclusion：In Tca8113 cells NOD2 expression was positively correlated with HBD-2 expression.NOD2 gene may promote the apoptosis,inhibit the proliferation of Tca8113 cells.