上海市某医院急性呼吸道感染儿童中人3型副流感病毒的检测及进化树分析

Detection and phylogenetic tree analysis of human parainfluenza virus type 3 in children with acute respiratory tract infection from a hospital in Shanghai

目的 了解3型副流感病毒（HPIV-3）的流行特点,以期为制定防制措施提供科学依据.方法 2009年6月到2010年6月上海交通大学医学院附属新华医院送上海市公共卫生临床中心检测的急性呼吸道感染患儿的鼻咽分泌物标本164份,反转录PCR法检测标本中的HPIV-3.对5份阳性标本的血凝素-神经氨酸酶（HN）基因编码区全长（1 719 bp）进行基因序列测定和进化分析.两组间比较采用精确卡方检验（双侧）.结果 164份急性呼吸道感染患儿标本中HPIV阳性数为70份,其中HPIV-3阳性23份,阳性率为32.86％.感染多发生于春、夏季,且多数HPIV-3感染发生在13～36个月的患儿中.基于HN基因建立的系统进化树将5株上海分离株和36株来自不同国家和地区的参考株序列分成3个进化群（A、B和C）.5株上海株和5株北京分离株归属于C3a群,上海株之间核苷酸和氨基酸的同源性分别为99.0％～99.5％和99.7％～100.0％.结论 HPIV-3在此次急性呼吸道感染患儿中所占比例较高,株系主要为C3a群,其进化很可能具有区域相关性.

Objective To understand the epidemiologic feature of human parainfluenza virus type 3 （HPIV-3） in Shanghai,and to provide scientific evidence for formulating prevention and control measures in the future.Methods A total of 164 nasopharyngeal aspirates samples taken from children with acute respiratory infection （ARI） were collected from Xinhua Hospital Affiliated to Shanghai Jiaotong University and sent to Shanghai Public Health Clinical Center from June 2009 to June 2010.Samples were detected for HPIV-3 by reversed transcription-polymerase chain reaction （RT-PCR）.Full-length hemagglutininneuraminidase （HN） gene （1 719 bp） of five positive samples were sequenced for phylogenetic analysis.Comparison between two groups was evaluated by the precise chi-square test （two sided）.Results Of 164 samples,70 samples were infected with parainfluenza virus,and HPIV-3 was detected positive in 23 samples with the positive rate of 32.86%.HPIV-3 infections were most common in spring and summer,and most of infections were mainly found in 13-36 month-old infants.Five Shanghai isolates and 36 reference sequences from different countries and areas were divided by HN gene-based phylogenetic tree into three clusters （A,B and C）.Five Shanghai isolates and five Beijing isolates belonged to C3a group.The homologies of nucleotide and amino acid sequences between five Shanghai isolates and five Beijing isolates were 99.0%-99.5% and 99.7%-100.0%,respectively.Conclusions HPIV-3 accounts for a high proportion in children with ARI in Shanghai.C3a group may be the main lineage of HPIV-3,which suggests that HPIV-3 may be of regionally correlation.