常压低氧小鼠氧耗量测定新装置的研究及应用

The construction and application of a novel apparatus for detecting oxygen consumption of mice under normobaric hypoxia

目的:建立一种实时记录常压低氧环境中动物氧耗量的方法。方法:本实验装置由动物舱、补水控制系统、天平、软管、装有体重记录软件的电脑等组成。为了实现常压低氧,用水补充动物消耗的氧气以保持动物舱内压力恒定,这个过程由气液联动装置控制;补充的水量由天平测量并同步输出信号至excel文档中。用注射器抽气校准方法检测了装置的准确性和精度。利用该装置观察了6只急性重复低氧小鼠(处理组)和6只未经低氧处理的小鼠(对照组)的常压低氧过程的氧耗量特征。结果:不同体积抽气量与相应补水量两组数据配对t检验P=1;重复抽1 ml氧气6次的补水量变异系数为4%。处理组小鼠的存活时间为(58.8±6.8)min,显著高于对照组(46.0±8.7)min(P<0.05)。处理组小鼠的总氧耗量为(85.1±8.5)ml,显著高于对照组(73.6±5.4)ml(P<0.05)。结论:处理组小鼠摄取氧总量增多从而显著延长其存活时间。氧耗量测定装置准确度和精密度较高,可用于低氧研究中氧耗量的测定。

Objective： To establish a method for real-time recording the oxygen consumption of mice under normobaric hypoxia. Melh- ods： The experimental apparatus was made up of animal container, filling water control system, electronic balance, hose, a computer with weight recording software, etc. The working principle was that the oxygen censumed by animal was replaced by water filling which was con- trolled by the pneumatic and hydraulic actuator. The water was weighted by an electronic balance and the weight signal was recorded into excel file at the same time. The accuracy and precision of the apparatus were detected by a 10 ml syringe. The oxygen consumption characteristics of 6 acute repetitive hypoxia mice and 6 normal mice were observed. Results： The P value for the paired t test was 1 and the CV value was 4%. The survival time and total oxygen consumption of acute repetitive hypoxia mice were both significantly increased compared to nomud mice （ P 〈 0.05）, which were （58.8 ± 6.8 ） min and （ 46.0 ± 8.7 ） min respectively for the survival time and （85.1 ± 8.5） ml and （ 73.6 ± 5.4） ml respectively for total oxygen consumption. Conclusion： The hypoxia tolerance of the acute repetitive hypoxia mice can significantly improved by taking more oxygen in the animal cabin. The accuracy and precision of the apparatus are high and it can be used for the determination of oxygen consumption in hypoxia research.