宫颈腺癌细胞与宫颈鳞癌细胞蛋白表达差异的质谱分析

Mass spectrometry analysis of proteins differentially expressed between adenocarcinoma and squamous carcinoma of the cervix

目的通过蛋白组学比较宫颈腺癌细胞与宫颈鳞癌细胞之间的蛋白表达差异,探讨宫颈腺癌预后不良与侵袭/转移率较高的原因。方法通过高效液相色谱与Orbitrap Elite/Velos Pro质谱联用对宫颈腺癌细胞系与宫颈鳞癌细胞系的蛋白组进行鉴定,然后通过生物信息学分析发现两种细胞系之间的差异表达蛋白。结果从两种细胞系中,共鉴定到2 373种高可信度蛋白,其中有787种差异表达蛋白。在腺癌细胞系高表达的蛋白中,有10.8%的蛋白与提高产能代谢相关;在腺癌细胞系低表达的蛋白中,有14.3%的蛋白与调节细胞凋亡相关。此外,细胞骨架蛋白在腺癌细胞系中呈现低表达。结论高产能代谢和低细胞凋亡可能是宫颈腺癌与鳞癌相比预后不良的重要原因,而细胞骨架不稳定性的增加可能导致腺癌更易发生侵袭/转移。

Objective To explore the mechanism for poor prognosis and high invasion /metastasis of cervical adenocarcinoma,and to compare the protein expression profiles of cervical adenocarcinoma cell line and squamous carcinoma cell line. Methods Differentially expressed proteins between cervical adenocarcinoma HeLa cells and cervical squamous carcinoma SiHa cells were identified by high performance liquid chromatography-Orbitrap Elite /Velos Pro mass spectrometry and analyzed by bioinformatics approaches. Results A total of 2 373 high-credibility proteins were identified in the two cell lines,and 787 of them were expressed differentially. Notably,10. 8% of the up-regulated proteins in the adenocarcinoma HeLa cells were related to energy-producing metabolism. On the other hand,14. 3% of the down-regulated proteins in the adenocarcinoma HeLa cells were related to apoptosis. In addition,cytoskeletal proteins were also down-regulated in the adenocarcinoma cell line. Conclusion High energy-productivity and low apoptosis may be the important mechanisms underlining poor prognosis in cervical adenocarcinoma. Further more,reduced cell cytoskeletal structure stability may be responsible for the invasive nature of adenocarcinoma.