自体颗粒状肋软骨移植的实验研究

Diced costal cartilage autografts: experimental study

目的观察自体颗粒状肋软骨移植于鼻背皮下后不同时间段的组织学变化。方法将15只3月龄的新西兰大白兔的肋软骨切成约1.0 mm×1.5 mm×1.5 mm的颗粒状并移植于各自鼻背皮下,于术后2、4、6个月分别处死5只,取出移植物制备组织切片,通过HE染色和Masson Trichrome染色观察软骨活性、骨化程度。结果颗粒状肋软骨未见明显吸收,周围结缔组织包裹,融合成一个整体,术后2、4、6个月有核软骨细胞占总软骨细胞百分率分别为48.16%、50.41%和43.38%,3组间数据差异有统计学意义(P<0.05);三阶段软骨组织骨化率平均值分别为32.72%、45.10%、48.94%,组间差异无统计学意义(P>0.05);余留软骨组织基质异染性评分平均值分别为1.29、2.22、2.65,组间差异无统计学意义(P>0.05)。结论颗粒状肋软骨能与周围组织很好融合并形成一整体,无明显吸收,软骨活性、骨化情况相对稳定。

Objective To compare histological changes of diced costal cartilage autografts in the subcutaneous tissue of dorsum nasi in different time periods. Methods Fifteen New Zealand white rabbits aged 3 months were used for the study. The costal cartilage was finely diced into 1.0 mm ×1.5 mm×1.5 mmcubes and placed in the subcutaneous tissue of dorsum nasi respectively. Five of the rabbits were killed in the 2nd,4th and 6th month respectively during post-operative period. The histological section of each specimen was prepared with hematoxylin-eosin and Masson trichrome staining and used to evaluate chondrocyte viability and the degree of ossification. Results There was not clear absorption of the diced costal cartilage grafts. Diced costal cartilage grafts were wrapped in the peripheral connective tissue. They integrated into a whole. The mean percentage of nucleated chondrocytes in the 2nd,4th and 6th month during post-operative period accounted for 48.16,50.41,43.38 percent respectively of the total cartilage, and the differences were statistically significant（P 〈0.05）. The mean percentages of subehondral ossifying tissue in the 2nd,4th and 6th month were 32.72,45. 10,48.94 percent, respectively, and the differences were not statistically significant（ P 〉 0.05 ）. The mean visual assessment scores concerning the metachromatism of cartilage matrix were 1.29,2.22 and 2.65 during these three stages,respectively, and the differences were not statistically significant（ P 〉 0.05）. Conclusions Diced costal cartilage autografts can be wrapped in the peripheral connective tissue well and form a unified whole. It is relatively stable interms of about the chondrocyte viability and the status of bone metaplasia in the diced costal cartilage grafts. There＇s no obvious absorption.