STZ小剂量多次与大剂量单次腹腔注射诱导糖尿病大鼠模型的研究

Research on Streptozotocin Induced Diabetic Rats by Low Dose Repeated and Large Dose with a Single Intraperitoneal Injection

目的观察小剂量STZ多次与大剂量单次腹腔注射诱导糖尿病大鼠模型的成模率,探讨STZ建立糖尿病动物模型的理想方案.方法健康雄性SD大鼠48只,随机分为6组,第1组(20 mg/kg)、第2组(30mg/kg)、第3组(40 mg/kg)、第4组(50 mg/kg)、第5组(60 mg/kg)和第6组(对照组),其中1、2、3组按多次腹腔注射方案,每次注射间隔时间为5 d,4、5组按一次腹腔注射方案.在每次注射后第5天进行血糖的测定.结果第2组注射4次后造模成功,第3组注射2次后造模成功,第4、5组在注射1次后造模均成功.至实验结束时,第2～5组造模成功率分别为75%、100%、75%、75%,第1组注射5次后造模仍未成功.第5组有2只大鼠死亡.结论一次腹腔注射STZ 50 mg/kg,成模率较高,存活率高,是建立SD大鼠糖尿病模型较为经济的方案.

Objective The study was aimed to observe the success rate of streptozotocin（STZ） induced diabetic rats by low dose repeated and large dose with a single intraperitoneal injection,and explore the ideal scheme of STZ induced diabetic rats. Methods 48 healthy male SD rats were randomly divided into 6 groups： group 1（20mg/kg）,group 2（30 mg/kg）,group 3（40 mg/kg）,group 4（50 mg/kg）,group 5（60 mg/kg） and group 6（the control group）. The groups 1-3 were treated with repeated intraperitoneal injection scheme,and each injection interval time was 5 days. The groups 4 and 5 were conducted according to the single intraperitoneal injection scheme.Blood glucose was measured at the fifth day after each injection. Results The rats model of group 2,group 3,and groups 4 and 5 was successfully established after four times, two times and one time of injection,respectively. At the end of the experiment,the success rate of groups 2 to 5 was 75%,100%,75% and 75%,respectively. In group 1, the model has not yet succeeded after five times injection. In group 5, 2 rats died. Conclusion The single intraperitoneal injection of STZ 50 mg/kg was an economical approach to induce diabetic SD rats, which has high success rate and survival rate.