水通道蛋白-4、基质金属蛋白酶-2,-14在胶质瘤瘤周水肿组织中的表达及其临床意义

Expressions of aquaporin-4, matrix metallo-proteinase-2 and matrix metallo-proteinase-14 in peritumor edematous zone of glioma and clinical implications

目的 对比不同恶性程度胶质瘤的瘤周水肿区与正常脑组织中基质金属蛋白和水通道蛋白的差别,探讨胶质瘤细胞侵袭能力与脑水肿之间的关系.方法 选取新乡市中心医院神经外科在2011年12月至2013年6月经手术病理证实的27例胶质细胞瘤病例标本,采用SP免疫组织化学方法对胶质瘤水肿组织和正常脑组织中水通道蛋白-4（AQP-4）、基质金属蛋白酶（MMP）-2、-14的表达进行测定.胶质瘤Ⅰ和Ⅱ级作为低度恶性组,胶质瘤Ⅲ和Ⅳ级作为高度恶性组,比较正常脑组织组与低度恶性组、高度恶性组之间的差异,同时分析AQP-4、MMP-2,-14之间的相关性.结果 AQP-4、MMP-2,-14随着胶质瘤病理级别的增高而升高,在正常脑组织组[分别为（117±12）、（102±11）、（106±11）IA]、低度恶性组[分别为（118±12）、（128±12）及（130±11）IA]和高度恶性组[分别为（151±19）、（152±9）及（156±13） IA]间的差异有统计学意义（P＜0.01）.结论 AQP4、MMP-2,-14在脑组织及各级胶质瘤瘤周水肿组织均表达呈正相关,提示胶质瘤的侵袭性与胶质瘤水肿程度呈正相关.

Objective To distinguish the expressions of matrix metallo-poteinase and aquaporin in peritumor edematous zone and normal brain tissue for different pathological levels of glioma and explore the relationship of glioma cell invasiveness and brain edema.Methods The immunohistochemical method of SP was employed to detect the expressions of aquaporin-4 （AQP-4）,matrix metallo-proteinase-2 （MMP-2） and matrix metallo-proteinase-14 （MMP-14） in glioma and normal brain tissue.Due to a rarity of glioma Grades Ⅰ and Ⅱ,grades Ⅰ and Ⅱ glioma were pooled into low malignancy group （LMG） and grades Ⅲ and Ⅳ into high malignancy group （HMG）.The software program SPSS 11.0 was used for Kolmogorov-Smirnov test of independent samplets.The differences were detected between normal brain tissue and LMG and HMG.Also the relationship of AQP4,MMP-2 and MMP-14 was analyzed.Results With the advancing pathological grades of glioma,the expression of AQP-4,MMP-2 and MMP-14 were higher in positive areas.There were significant deviations among 3 groups.Conclusion The expressions of AQP-4,MMP-2 and MMP-14 in normal brain tissue and all levels of glioma edema are positively correlated.And there is a close correlation between glioma invasiveness and edema extent.