抑制阿萨希毛孢子菌顶体对其增殖及致病性的影响

Effects of inhibiting Spitzenkrper formation on proliferation and murine pathogenicity of T. asahii

目的 探讨抑制阿萨希毛孢子菌（Trichosporon asahii,Zasahii）顶体（Spitzenkorper）形成后对其细胞增殖及致病性的影响.方法 经不同浓度细胞松弛素D（0.5、1.0及2.0 μmol/L）处理阿萨希毛孢子菌,以抑制其顶体形成,体外测定生长曲线及生长菌落变化;小鼠体内接种上述菌悬液后,统计小鼠3周内的死亡率,并取内脏进行组织培养和病理检查,统计感染率.结果 经细胞松弛素D处理抑制顶体形成后,Z asahii细胞生长明显延迟8～16 h,生长菌落直径明显变小;小鼠死亡率及感染率亦出现不同程度降低,并随着药物浓度的升高,其致病性明显降低.结论 抑制阿萨希毛孢子菌顶体形成后,细胞增殖明显受到抑制,且致病性也随细胞松弛素D浓度的增加而降低.提示顶体可以作为研制新型抗真菌药物的新靶点.

Objective To investigate the effects of proliferation and pathogencity of T.asahii in murine model after inhibititing spitzenk（o）rper formation.Methods The suspension of T.asahii were dealed with different concentration of Cytochalasin D for inhibiting Spitzenk（o）rper formation.In vitro,each suspension was measured in the Growth Curve and colony.In vivo,the different suspensions were inoculated into ten mice through caudal vein respectively,and then the death rate was counted within three weeks.Meanwhile the main viscera of the mice were examined by mycologic culture and pathology and finally the infection rate were counted.Results Compared with the control group,the speed of fungal growth in the experimental group were prolonged about 8 ～ 16 h obviously and the colony on culture medium were also minished.The death rate and infection rate of mice were accordingly decreased with the increasing concentration of Cytochalasin D.In other words,pathogencity of T.asahii was clearly cut down.Conclusions When the Spitzenk（o）rper formation being inhibited by Cytochalasin D,the proliferation of T.asahii was restricted and the pathogencity decreased apparently.With the increasing of density of Cytochalasin D,the pathogencity of mice decreased gradiently.It suggested that there might be a clue of a new anti-fungal target.