NVP-BEZ235对肝癌HepG2细胞增殖、凋亡及侵袭转移的影响

Effect of NVP-BEZ235 on proliferation,apoptosis,invasion and metastasis of HepG2 cells

目的探讨PI3K/Akt/mTOR双靶点抑制剂NVP-BEZ235在体外对肝癌HepG2细胞增殖、凋亡及侵袭转移的影响。方法采用0、0.01、0.1、1.0μmol/L NVP-BEZ235处理HepG2细胞,四甲基偶氮唑盐(MTT)比色法检测不同浓度NVP-BEZ235处理24、48、72和96h的增殖抑制率,流式细胞仪、Transwell侵袭实验、荧光定量PCR及免疫印迹检测不同浓度NVP-BEZ235处理48h后的细胞周期分布、穿膜细胞数及基质金属蛋白酶(MMP)-2的mRNA和蛋白水平。结果 NVPBEZ235可呈剂量和时间依赖的方式升高增殖抑制率(P<0.05);除0.01μmol/L处理24h的晚期凋亡率外,0.01、0.1、1.0μmol/L NVP-BEZ235处理24、48h的早、晚期凋亡率均高于0μmol/L(P<0.05);0.01、0.1、1.0μmol/L NVP-BEZ235处理48h的G0/G1期细胞比例高于0μmol/L,穿膜细胞数、MMP-2 mRNA和蛋白水平及S期和G2/M期细胞比例均低于0μmol/L(P<0.05),且各浓度间的差异均有统计学意义(P<0.05)。结论 NVP-BEZ235可抑制肝癌HepG2细胞增殖及侵袭转移,促进其凋亡和阻滞细胞在G0/G1期并降低MMP-2表达。

Objective To explore the effect of NVP-BEZ235 on the proliferation, apoptosis, invasion and metastasis of HepG2 Cells. Methods The HepG2 cells were treated with different concentrations of NVP-BEZ235（0, 0.01, 0.1, 1.0μmol/L）. MTT was used to study the proliferation inhibition rates at 24, 48, 72 and 96h treated with different concentrations of NVP-BEZ235. Transwell assay was used to check the invasion and metastasis of HepG2 cells with NVP-BEZ235. The cycle distribution at 48h after treatment with NVP-BEZ235 was detected by flow cytometry. The RT-PCR and Western blotting were used to measure the change of MMP-2 expression. Results NVP-BEZ235 can increase the proliferation inhibition rates of HepG2 cell in a dose-and time-dependent manner. In addition to the late apoptosis rate of 0.01μmol/L at 24h, the early and late apoptosis rates of the remaining concentrations were higher than those of 0μmo/L at 24h and 48h（P〈0.05）. The proportion of cells in G0/G1 phase of 0.01, 0.1 and 1.0μmol/L NVP-BEZ235 were higher than those of 0μmo/L, while the transmembrane cell number, the proportion of cells in G2/M and S phase, and the protein and mRNA levels of MMP-2 of 0.01, 0.1 and 1.0μmol/L NVP-BEZ235 were lower than those of 0μmo/L at 48h（ P〈0.05） . Conclusion NVP-BEZ235 effectively inhibits the abilities of proliferation, invasion and metastasis abilities of HepG2 cells, promotes the apoptosis, arrests cells at G0/G1 phase and decreases the expression of MMP-2.