半夏泻心汤对幽门螺杆菌感染胃炎小鼠胃内微生物及酶的影响

Effect of Banxia Xiexin decoction on gastrointestinal microbiota and enzyme activities in mice with Helicobacter pylori associated gastritis

目的:探讨半夏泻心汤对幽门螺杆菌(Helicobacter pylori,H.pylori)感染胃炎小鼠的疗效与胃肠道微生物及酶活性的关系,从微生态学角度研究半夏泻心汤的疗效机制.方法:通过建立H.pylori感染胃炎小鼠模型,随机分为正常组、模型组、克拉霉素组、半夏泻心汤组,分别灌胃给药克拉霉素、半夏泻心汤,分析胃内菌群数及酶活性.结果:模型组胃内的细菌数、大肠杆菌数、双歧杆菌数、乳酸菌数以及蛋白酶活性均高于正常组(P<0.05或P<0.01),纤维素酶及淀粉酶活性均低于正常组(P<0.05或P<0.01);经克拉霉素治疗后的模型小鼠细菌数、双歧杆菌数、乳酸菌数及纤维素酶酶活性均低于正常组(P<0.05或P<0.01);经半夏泻心汤治疗后,细菌数虽有所降低,但仍高于正常组,双歧杆菌和乳酸菌增长十分迅速,远高于其他各组(P<0.01),纤维素酶及淀粉酶已恢复至正常(P>0.05).结论:半夏泻心汤能显著调节H.pylori感染胃炎小鼠胃内微生物及酶活性.

AIM： To explore the relationship between the therapeutic effect of Banxia Xiexin decoction on Helicobacter pylori （H. pylori） associated gastritis and gastrointestinal microbiota and enzyme activities. METHODS： A mouse model H. pylori associ- ated gastritis was established, and the effects of clarithromycin and Banxia Xiexin decoction on the rnicrobiota and enzyme activities were analyzed. RESULTS： The numbers of bacteria, Escherichia coli, lactobacillus and protease activities in mice with H. pylori associated gastritis were signifi- cantly higher than those in control mice （P 〈 0.05 or P 〈 0.01）, while cellulase and amylase activi- ties were significantly lower in model mice than in control mice （P 〈 0.05 or P 〈 0.01）. The num- bers of bacteria, Escherichia coli, lactobacillus and bifidobacteria in clarithromycin-treated mice were significantly lower than those in control mice （P 〈 0.05 or P 〈 0.01）. After Banxia Xiexin decoction treatment, the numbers of bacteria became lower, but were still higher than those in control mice. Bifidobacteria and lactobacillus in Banxia Xiexin decoction-treated mice grew very rapidly, and their numbers were much higher than those in the other groups （P 〈 0.01）. Amylase and cellulase activities in Banxia Xiexin decoction-treated mice returned to normal levels （P 〉 0.05）. CONCLUSION： Banxia Xiexin decoction can significantly adjust gastrointestinal microbiotia and enzyme activities in mice with H. pylori associ- ated gastritis.