网织红细胞各参数的检测在贫血性疾病中的临床意义

Clinical significance of reticulocyte-related parameters determination in anemic diseases

目的探讨网织红细胞(RET)相关参数检测在贫血性疾病中的临床意义。方法应用Sysmex XE-5000全自动血液分析仪对患者的RET百分比(RET%)、RET绝对值(RET#)、高荧光强度网织红细胞百分比(HFR%)、中荧光强度网织红细胞百分比(MFR%)、低荧光强度网织红细胞百分比(LFR%)以及未成熟网织红细胞比率(IRF)进行检测,并与对照组进行比较分析。结果溶血性贫血患者LFR%降低,RET%、RET#、HFR%、MFR%以及IRF均比对照组明显升高,差异有统计学意义(P<0.01);再生障碍性贫血患者RET%、RET#、HFR%、MFR%、LFR%以及IRF明显降低,差异有统计学意义(P<0.01);缺铁性贫血患者RET%、RET#、HFR%、MFR%、LFR%以及IRF比对照组稍增高,但差异无统计学意义。结论网织红细胞相关参数检测可以更准确反映网织红细胞的成熟状态,从而判断骨髓的造血功能,对不同类型的贫血性疾病的诊断、治疗具有重要参考价值。

Objective To explore the clinical significance of reticulocyte （RET ）-related parameters determination in anemia diagnosis .Methods Reticulocyte percentage （RET% ） ,RET count （RET#） ,percent of high fluores-cent reticulocyte （LFR% ） ,percent of middle fluorescent reticulocyte （MFR% ） ,percent of low fluorescent reticulocyte （HFR% ） ,and immature RET fraction （IRF） of patients were determined by Sysmex XE-5000 automated blood analyzer and compared with the control group .Results LFR% of patients suffering from hemolytic anemia decreases ,RET% ,RET # ,HFR% ,MFR% and IRF of patients suffering hemolytic anemia were obviously higher than those of normal control group and the difference is quite significant （P〈 0 .01）;while RET% ,RET # ,HFR% , MFR% ,LFR% and IRF of patients with aplastic anemia were significantly reduced （P〈 0 .01）;RET% ,RET # , HFR% ,MFR% ,LFR% and IRF of patients suffering iron-deficiency anemia were slightly higher than those of normal control group ,but there were no significant differences among them .Conclusion RET-related parameters are able to reflect the mature of reticulocyte more accurately ,so it can evaluate the hematopoiesis of the bone marrow and it present an important value in the diagnosis and treatment of different types of anemia .