邻苯二甲酸单丁酯致小鼠肝脏和肾脏组织的氧化损伤

Oxidative Stress on Liver and Kidney of Mice Induced by Mono-n-butyl Phthalate

为了探究邻苯二甲酸单丁酯(MBP)对小鼠肝脏和肾脏的氧化损伤,将42只BALB/c小鼠随机分为7组,每组6只,分别为25mg·kg-1、50mg·kg-1、100mg·kg-1、200mg·kg-1的4个MBP染毒组、1个100mg·kg-1的邻苯二甲酸二丁酯(DBP)染毒组、1个空白对照组、1个溶剂对照组。染毒期间对小鼠的体征进行观察;14d后取其肝脏和肾脏组织,制作小鼠肝脏和肾脏组织的切片,对肝脏和肾脏的组织学形态进行观察;制作组织匀浆液用于检测肝脏和肾脏组织细胞的ROS、GSH、MDA的含量,以了解MBP对肝脏和肾脏组织的氧化损伤作用。结果显示:各剂量组小鼠肝细胞和肾小管上皮细胞均出现不同程度的细胞核固缩、细胞水肿、空泡样变、脂肪滴增大融合等症状;ROS和MDA的含量与MBP的染毒剂量呈正相关,GSH的含量与MBP的染毒剂量呈负相关;相同剂量的DBP与MBP染毒组相比,MBP染毒组的ROS和MDA含量较高、GSH含量较低。表明,MBP的暴露与小鼠肝脏和肾脏组织的氧化损伤存在直接联系。

To investigate the potential oxidative stress of mono-n-butyl phthalate（MBP）on liver and kidney of mice,42 BALB/c mice were randomly divided into 7 groups,with 6 mice in each group,4 groups were exposed to MBP by gavage for 14 d at gradient doses of 25 mg·kg^-1 ,50 mg·kg^-1 ,100 mg·kg^-1 and 200 mg· kg^-1 ,one group was exposed to DBP by gavage for 14 d at a dose of 100 mg·kg^-1 ,a control group,a vehicle control group.The infected mice signs were observed during the research.The liver and kidney slices were prepared to observe the histological changes after 14 d.The contents of ROS,GSH and MDA in the liver and kidney homogenate were measured to indicate the oxidative stress.The results showed that,the liver and kidney histological changes were observed in MBP groups,light microscope observation showed hepatocytes and renal tubular cells were karyopyknosis,swelling,ballooned changing and fatty droplets clustering.It also showed that the contents of ROS and MDA increased gradually while GSH content decreased with the increase of exposure dose,and all the studied biomarkers changed in dose-response manners.Under the same concentration,the contents of ROS and MDA in MBP exposed groups were all higher than those of DBP infected groups,but the content of GSH decreased.It was demonstrated that MBP could increase the oxidative stress and cause oxidative stress to liver and kidney of mice at relatively high dose.Together,there is a possible relationship between MBP exposure and oxidative stress on liver and kidney of mice.