稻瘟病菌MoYpt5蛋白互作网络预测

The Prediction of Protein-protein Interaction Network of MoYpt5 in Magnaporthe oryzae

稻瘟病菌(Magnaporthe oryzae)共有11个假定的Rab蛋白家族成员,本文选取了MoYpt51(MGG_06241)和MoYpt52(MGG_01185)进行了生物信息学分析。通过搜索多个大型蛋白互作数据库和文献,共得到数百个与核心蛋白互作的蛋白和互作对。利用信息处理技术和高效制图平台将这些蛋白互作对构建成互作网络,得到若干个具有生物学意义的模块。结果表明,筛选得到的互作蛋白中,有的参与了蛋白降解的泛素途径(MGG_04053等)、囊泡介导的蛋白胞内运输(MGG_01238等),有的在蛋白、染色体的组装和修饰等过程(MGG_03677等)起重要作用。大部分假定互作蛋白定位于细胞质和质膜上,为其与目标蛋白互作提供了空间可能性。

There are 11 putative members of Rab family in Magnaporthe oryzae. MoYpt51 （MGG_06241） and MoYpt52 （MGG_01185） were chosen to conduct analysis of their protein interaction network by bioinformatics. We acquired hundreds of proteins and interaction-partners which interact with the core protein by searching several large protein interaction databases and references, and got a few significantly biological modules on the base of the technology of bioinformatics analysis and the platform of highly effective charting. The results showed that some （MGG_04053, etc.） of the screened proteins involve in the ubiquitin pathway of protein degradation and the protein transportation processes introduced by vesicles in cells （MGG_01238, etc.）, some （MGG_03677, etc.） play a crucial role in the assembling and modifying processes of proteins and chromosomes. Most of the interaction proteins are localized in the cytoplasm and on the cell membrane which generate a spatial possibility of interacting with targeting proteins.