不同采收期引种柴胡不同部位总皂苷和皂苷a、d含量的研究

Study on the Content of Saikosaponins and Saikosaponin a, d of Different Parts from Introduction Bupleurum chinense in Various Collecting Periods

以引种到唐山的北柴胡为样本,研究其不同部位总皂苷和皂苷a、d含量的动态变化规律,探讨引种柴胡的最适采收期。用紫外分光光度法和HPLC法分别测定不同采收期柴胡总皂苷及皂苷a、d的含量。根茎叶中均含有皂苷,整个生长期内皂苷含量呈上下波动,但总体呈下降趋势;不同部位不同采收期均存在明显差异,根中高于茎和叶,茎中含量最少;根中皂苷d含量均高于皂苷a;总皂苷和皂苷a、d含量在种植第1年10月含量最高,第2年9月含量最低,皂苷a、d含量第2年8月含量次高。紫外分光光度法和HPLC法分别测定柴胡总皂苷及皂苷a、d方法可行,效果良好。确定了引种柴胡最适采收时期,为引种柴胡药材的采收及生产提供了一定科学依据。

The research took Tangshan introduction of Bupleurum chinense as the sample to study the dynamic change law of saikosaponins and saikosaponin a, d of different parts and discuss the optimal harvest time of Bupleurum chinense. The content of saikosaponins and saikosaponin a, d was determined by UV-visible spectropholometry and HPLC method. The root, stem and leaf contained saikosaponin, the content of saikosaponin in the whole growth period was fluctuated, but the overall trend was on declining. There were obvious difference in different parts and different harvest time, the content in root was more than that in stem and leaf. The content in stem was the lowest. The content of saikosaponin d in root were higher than that of saikosaponin a; the content of saikosaponins and saikosaponin a, d was the highest in October of the first year, and was the lowest in September of the second year, the content of saikosaponin a and d was higher in August. The determination methods of saikosaponins by UV-visible spectrophotometry and saikosaponin a and d by HPLC was proved to be feasible and the result was good. The optimal harvest period of introduction Bupleurum chinense was determined, which could provide scientific basis for harvesting and production.