福建及邻近省份禽源多杀性巴氏杆菌的分离鉴定

CHARACTERIZATION OF AVIAN PASTEURELLA MULTOCIDA ISOLATES FROM FUJIAN AND ITS NEIGHBOR PROVINCES

本研究收集2007-2013年福建省及邻近省份的疑似禽霍乱病死亡鸡、鸭、鹅，进行细菌分离，PCR进行种和荚膜群的鉴定，琼脂扩散试验鉴定其Heddleston氏耐热菌体抗原型。结果共分离鉴定多杀性巴氏杆菌95株，其中鸭源74株，鸡源17株，鹅源4株。其荚膜群全为A型，1型耐热菌体抗原型占67.4%。与20世纪80年代以来我国已有的报道相同，禽源（不含火鸡）多杀性巴氏杆菌血清型仍然以A：1为主。

Dead chickens, ducks and geese suspected of fowl cholera were collected in 2007-2013 from Fujian and its neighbor provinces and subject to isolation of Pasteurellamultocida. A total of 95 Pasteurellamultocida isolates were obtained, among which 74 isolates were isolated from ducks, 17 from chickens and 4 from geese. These bacterial strains were then determined for species and capsular types in PCR and for heat-stable somatic antigens in agar diffusion test. All isolates belonged to capsular type A and 64 isolates to heat-stable somatic antigens 1. These results have indicated that the main serotype of Pasteurellamultocida isolates from avian （excluding turkey） in China has been remained A：1 since 1980’s.