2012-2013年山东省禽源大肠杆菌中质粒介导喹诺酮类药物耐药基因的检测

DETECTION OF PLASMID-MEDIATED QUINOLONE RESISTANCE GENES IN AVIAN ESCHERICHIA COLI ISOLATES FROM SHANDONG PROVINCE IN 2012-2013

为研究近年来山东省禽源致病性大肠杆菌中质粒介导喹诺酮类药物耐药（plasmid-mediated quinolone resistance， PMQR）基因的基因型分布，及其对喹诺酮类抗生素的耐药性的影响，分别采用针对qnrA、qnrB、qnrC、qnrD、qnrS、oqxA、oqxB与qepA 8个耐药基因的通用引物，对93株2012-2013年分离自山东省的禽源大肠杆菌进行PCR检测，并对其进行了5种喹诺酮类药物的药敏试验。结果表明山东省禽源大肠杆菌对5种喹诺酮类抗生素均产生了较高耐药性（50.54%-86.30%）；PMQR基因携带率达到60.21%（56/93），其中26.88%（25/93）的菌株携带2种PMQR基因，1.07%（1/93）的菌株携带3种PMQR基因；qnrA、qnrB、qnrC、qnrD与qepA基因未被检测到，qnrS、oqxA和oqxB基因在山东省禽源致病性大肠杆菌中分布较为广泛，其检出率依次为22.58%（21/93）、40.86%（38/93）和24.73%（23/93）。

To investigate the distribution of plasmid-mediated quinolone resistance （PMQR） genes and their effect on the bacterial resistance to the quinolone antibiotics in recent years, qnrA, qnrB, qnrC, qnrD, qnrS, oqxA, oqxB and qepA genes of 93 avian E. coli strains isolated from Shandong Province were detected individually in PCR, and the resistance to Quinolones （Ciprofloxacin, Lomefloxacin, Ofloxacin, Norfloxacin, Pefloxacin） was detected in antimicrobial susceptibility test. Among 93 E.coli isolates tested, 47 （50.54%） to 80 （86.30%） strains were resistant to five Quinolones respectively, and 56 （60.21%） strains were positive to PMQR genes. In addition, 25 isolates （26.88%） harbored two kinds of PMQR genes and 1 isolates （1.07%） harbored three kinds of PMQR genes. The positive rates of qnrS, oqxA and oqxB gene were 22.58%（21/93）, 40.86%（38/93） and 24.73%（23/93） , respectively. None of qnrA, qnrB, qnrC, qnrD and qepAgenes was detected.